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Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii

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Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii

dc.contributor.author	Belloch, Carmela
dc.contributor.author	Pérez Torrado, Roberto
dc.contributor.author	González, Sara S.
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	García Martínez, José
dc.contributor.author	Querol Simón, Amparo
dc.contributor.author	Barrio Esparducer, Eladio
dc.date.accessioned	2013-12-02T13:23:07Z
dc.date.available	2013-12-02T13:23:07Z
dc.date.issued	2009
dc.identifier.citation	Belloch, Carmela Pérez Torrado, Roberto González, Sara S. Pérez Ortín, José Enrique García Martínez, José Querol Simón, Amparo Barrio Esparducer, Eladio 2009 Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii Applied and Environmental Microbiology 75 8 2534 2544
dc.identifier.uri	http://hdl.handle.net/10550/31687
dc.description.abstract	Recently, a new type of hybrid resulting from the hybridization between Saccharomyces cerevisiae and Saccharomyces kudriavzevii was described. These strains exhibit physiological properties of potential biotechnological interest. A preliminary characterization of these hybrids showed a trend to reduce the S. kudriavzevii fraction of the hybrid genome. We characterized the genomic constitution of several wine S. cerevisiae × S. kudriavzevii strains by using a combined approach based on the restriction fragment length polymorphism analysis of gene regions, comparative genome hybridizations with S. cerevisiae DNA arrays, ploidy analysis, and gene dose determination by quantitative real-time PCR. The high similarity in the genome structures of the S. cerevisiae × S. kudriavzevii hybrids under study indicates that they originated from a single hybridization event. After hybridization, the hybrid genome underwent extensive chromosomal rearrangements, including chromosome losses and the generation of chimeric chromosomes by the nonreciprocal recombination between homeologous chromosomes. These nonreciprocal recombinations between homeologous chromosomes occurred in highly conserved regions, such as Ty long terminal repeats (LTRs), rRNA regions, and conserved protein-coding genes. This study supports the hypothesis that chimeric chromosomes may have been generated by a mechanism similar to the recombination-mediated chromosome loss acting during meiosis in Saccharomyces hybrids. As a result of the selective processes acting during fermentation, hybrid genomes maintained the S. cerevisiae genome but reduced the S. kudriavzevii fraction.
dc.relation.ispartof	Applied and Environmental Microbiology, 2009, vol. 75, num. 8, p. 2534-2544
dc.subject	Genètica molecular
dc.subject	Saccharomyces
dc.subject	Saccharomyces cerevisiae
dc.title	Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
dc.type	journal article	es_ES
dc.date.updated	2013-12-02T13:23:07Z
dc.identifier.doi	10.1128/AEM.02282-08
dc.identifier.idgrec	048855
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1128/AEM.02282-08