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Downregulation of KLF8 expression by shRNA induces inhibition of cell proliferation in CAL27 human oral cancer cells

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Downregulation of KLF8 expression by shRNA induces inhibition of cell proliferation in CAL27 human oral cancer cells

dc.contributor.author	Bin, Zhang	es
dc.contributor.author	Ke-Yi, Li	es
dc.contributor.author	Wei-Feng, Zhang	es
dc.contributor.author	Li-Cheng, Jiang	es
dc.contributor.author	Xian-Bin, Liu	es
dc.contributor.author	Chun-Peng, Xia	es
dc.contributor.author	Dao-Ying, Yuan	es
dc.contributor.author	Shu-Wei, Liu	es
dc.date.accessioned	2014-05-29T07:11:35Z
dc.date.available	2014-05-29T07:11:35Z
dc.date.issued	2013	es
dc.identifier.citation	Bin, Zhang ; Ke-Yi, Li ; Wei-Feng, Zhang ; Li-Cheng, Jiang ; Xian-Bin, Liu ; Chun-Peng, Xia ; Dao-Ying, Yuan ; Shu-Wei, Liu. Downregulation of KLF8 expression by shRNA induces inhibition of cell proliferation in CAL27 human oral cancer cells. En: Medicina oral, patología oral y cirugía bucal. Ed inglesa, 2013, Vol. 18, No. 4: 591-596	es
dc.identifier.uri	http://hdl.handle.net/10550/35655
dc.description.abstract	Objectives: KLF8 is a member of KLF transcription factors which play an important tolr in oncogenesis. It is barely expressed in normal human epithelial cells but highly overexpressed in several types of human cancer cell lines. In the present study, we investigate the role of KLF8 in oral cancer and the effects of KLF8 knockdown via lentivirus mediated siRNA infection in human adenosquamos carcinoma CAL 27 cells. Study Design: We developed a vector-based siRNA expression system that can induce RNAi in CAL 27 oral cancer cells. Downregulation of KLF8 was confirmed by evaluating GFP expressions, RT-PCR and western blot analysis. Finally, the effects of KLF8 downregulation were analyzed by MTT assay and colony formation assays. Results: The expression levels of KLF8 mRNA and proteins are reduced in CAL 27 cells that transfected with 21- nt siRNA against KLF8. Lentivirus-mediated silencing of KLF8 reduces cell proliferation and colonies number, thereby indicating the role of KLF8 in cell proliferation and tumorigenesis. Conclusions: These results strongly suggest that KLF8 is essential for growth of CAL 27 cancer cells. A better understanding of KLF8 function and processing may provide novel insights into the clinical therapy of oral cancer.	en_US
dc.subject	Odontología	es
dc.subject	Ciencias de la salud	es
dc.title	Downregulation of KLF8 expression by shRNA induces inhibition of cell proliferation in CAL27 human oral cancer cells	es
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	es
dc.type.hasVersion	VoR	es_ES