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Metabolic Profile of chronic liver disease by NMR spectroscopy of human biopsies

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Metabolic Profile of chronic liver disease by NMR spectroscopy of human biopsies

dc.contributor.author	Martínez Granados, Beatriz
dc.contributor.author	Morales Tatay, José Manuel
dc.contributor.author	Rodrigo, José Manuel
dc.contributor.author	del Olmo, Juan
dc.contributor.author	Serra, Miguel Angel
dc.contributor.author	Ferrández Izquierdo, Antonio
dc.contributor.author	Celda Muñoz, Bernardo
dc.contributor.author	Monleon Salvado, Daniel
dc.date.accessioned	2015-02-28T12:16:33Z
dc.date.available	2015-02-28T12:16:33Z
dc.date.issued	2011
dc.identifier.citation	Martínez Granados, Beatriz Morales, José Manuel Rodrigo, José Manuel del Olmo, Juan Serra, Miguel Angel Ferrández, Antonio Celda, Bernardo Monleón, Daniel 2011 Metabolic Profile of chronic liver disease by NMR spectroscopy of human biopsies International Journal of Molecular Medicine 27 1 111 117
dc.identifier.uri	http://hdl.handle.net/10550/42541
dc.description.abstract	Abstract Among the different processes occurring during the evolution of liver disease, fibrosis has a predominant role. Liver fibrosis mechanisms are fairly constant irrespective of the underlying etiology. Cirrhosis is the end-stage of this reaction. Metabolic profiles, which are affected by many physiological and pathological processes, may provide further insight into the metabolic consequences of this severe liver disease. The aim of this study was to demonstrate the applicability of 1H high resolution magic angle spinning (HR-MAS) NMR spectroscopy in the biochemical profile determination of human liver needle biopsy samples for the characterization of metabolic alterations related to the severity of liver disease. We recorded and analyzed HR-MAS spectra of 68 liver tissue samples obtained by needle biopsy from patients with chronic liver disease. Multivariate analysis was applied to these data to obtain discrimination patterns and to reveal relevant metabolites. The metabolic characterization of liver tissue from needle biopsies by HR-MAS NMR spectroscopy provided differential patterns for cirrhotic and non-cirrhotic chronic liver disease tissue. Metabolites closely related to the liver metabolism such as some fatty acids, glucose and amino acids show differences between the two groups. Phospholipid precursors, which have been previously correlated with hepatic lesions also show differences. Furthermore, the correlation between histologically assessed liver disease stages and the levels of the most discriminative metabolites show that liver dysfunction is present at the initial stages of chronic hepatic lesions. Overall, this work suggests that the additional information obtained by NMR metabolomics applied to needle biopsies of human liver may be useful for assessing metabolic alterations and liver dysfunction in chronic liver disease.
dc.language.iso	eng
dc.relation.ispartof	International Journal of Molecular Medicine, 2011, vol. 27, num. 1, p. 111-117
dc.subject	Medicina
dc.subject	Patologia
dc.title	Metabolic Profile of chronic liver disease by NMR spectroscopy of human biopsies
dc.type	journal article	es_ES
dc.date.updated	2015-02-28T12:16:34Z
dc.identifier.idgrec	061167
dc.rights.accessRights	open access	es_ES