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Comparative transcriptomic analysis reveals similarities and dissimilarities in saccharomyces cerevisiae wine strains response to nitrogen availability

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Comparative transcriptomic analysis reveals similarities and dissimilarities in saccharomyces cerevisiae wine strains response to nitrogen availability

dc.contributor.author	Barbosa, Catarina
dc.contributor.author	García Martínez, José
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Mendes Ferreira, A.
dc.date.accessioned	2015-04-29T11:12:33Z
dc.date.available	2015-04-29T11:12:33Z
dc.date.issued	2015
dc.identifier.citation	Barbosa, Catarina García Martínez, José Pérez Ortín, José Enrique Mendes Ferreira, A. 2015 Comparative transcriptomic analysis reveals similarities and dissimilarities in saccharomyces cerevisiae wine strains response to nitrogen availability Plos One 10 4 e0122709
dc.identifier.uri	http://hdl.handle.net/10550/43520
dc.description.abstract	Nitrogen levels in grape-juices are of major importance in winemaking ensuring adequate yeast growth and fermentation performance. Here we used a comparative transcriptome analysis to uncover wine yeasts responses to nitrogen availability during fermentation. Gene expression was assessed in three genetically and phenotypically divergent commercial wine strains (CEG, VL1 and QA23), under low (67 mg/L) and high nitrogen (670 mg/L) regimes, at three time points during fermentation (12h, 24h and 96h). Two-way ANOVA analysis of each fermentation condition led to the identification of genes whose expression was dependent on strain, fermentation stage and on the interaction of both factors. The high fermenter yeast strain QA23 was more clearly distinct from the other two strains, by differential expression of genes involved in flocculation, mitochondrial functions, energy generation and protein folding and stabilization. For all strains, higher transcriptional variability due to fermentation stage was seen in the high nitrogen fermentations. A positive correlation between maximum fermentation rate and the expression of genes involved in stress response was observed. The finding of common genes correlated with both fermentation activity and nitrogen up-take underlies the role of nitrogen on yeast fermentative fitness. The comparative analysis of genes differentially expressed between both fermentation conditions at 12h, where the main difference was the level of nitrogen available, showed the highest variability amongst strains revealing strain-specific responses. Nevertheless, we were able to identify a small set of genes whose expression profiles can quantitatively assess the common response of the yeast strains to varying nitrogen conditions. The use of three contrasting yeast strains in gene expression analysis prompts the identification of more reliable, accurate and reproducible biomarkers that will facilitate the diagnosis of deficiency of this nutrient in the grape-musts and the development of strategies to optimize yeast performance in industrial fermentations.
dc.language.iso	eng
dc.relation.ispartof	Plos One, 2015, vol. 10, num. 4, p. e0122709
dc.subject	Transcripció genètica
dc.subject	Saccharomyces cerevisiae
dc.title	Comparative transcriptomic analysis reveals similarities and dissimilarities in saccharomyces cerevisiae wine strains response to nitrogen availability
dc.type	journal article	es_ES
dc.date.updated	2015-04-29T11:12:33Z
dc.identifier.doi	10.1371/journal.pone.0122709
dc.identifier.idgrec	102389
dc.rights.accessRights	open access	es_ES