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Functional consequences of microbial shifts in the human gastrointestinal tract linked to antibiotic treatment and obesity

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Functional consequences of microbial shifts in the human gastrointestinal tract linked to antibiotic treatment and obesity

dc.contributor.author	Hernández, Ester	es_ES
dc.contributor.author	Bargiela, Rafael	es_ES
dc.contributor.author	Suárez Díez, María	es_ES
dc.contributor.author	Friedrichs, Anette	es_ES
dc.contributor.author	Pérez-Cobas, Ana Elena	es_ES
dc.contributor.author	Gosalbes Soler, María José	es_ES
dc.contributor.author	Knecht, Henrik	es_ES
dc.contributor.author	Martínez-Martínez, Mónica	es_ES
dc.contributor.author	Seifert, Jana	es_ES
dc.contributor.author	von Bergen, Martin	es_ES
dc.contributor.author	Artacho, Alejandro	es_ES
dc.contributor.author	Ruiz, Alicia	es_ES
dc.contributor.author	Campoy, Cristina	es_ES
dc.contributor.author	Latorre, Amparo	es_ES
dc.contributor.author	Ott, Stephan J.	es_ES
dc.contributor.author	Moya, Andrés	es_ES
dc.contributor.author	Suárez, Antonio	es_ES
dc.contributor.author	Martins dos Santos, Vitor A. P.	es_ES
dc.contributor.author	Ferrer, Manuel	es_ES
dc.date.accessioned	2015-06-18T12:29:21Z
dc.date.available	2015-06-18T12:29:21Z
dc.date.issued	2013	es_ES
dc.identifier.citation	Gut Microbes Vol. 4 Issue 4: pp. 306-315	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44473
dc.description.abstract	The microbiomes in the gastrointestinal tract (GIT) of individuals receiving antibiotics and those in obese subjects undergo compositional shifts, the metabolic effects and linkages of which are not clearly understood. Herein, we set to gain insight into these effects, particularly with regard to carbohydrate metabolism, and to contribute to unravel the underlying mechanisms and consequences for health conditions. We measured the activity level of GIT carbohydrate-active enzymes toward 23 distinct sugars in adults patients (n = 2) receiving 14-d β-lactam therapy and in obese (n = 7) and lean (n = 5) adolescents. We observed that both 14 d antibiotic-treated and obese subjects showed higher and less balanced sugar anabolic capacities, with 40% carbohydrates being preferentially processed as compared with non-treated and lean patients. Metaproteome-wide metabolic reconstructions confirmed that the impaired utilization of sugars propagated throughout the pentose phosphate metabolism, which had adverse consequences for the metabolic status of the GIT microbiota. The results point to an age-independent positive association between GIT glycosidase activity and the body mass index, fasting blood glucose and insulin resistance (r2 ≥ 0.95). Moreover, antibiotics altered the active fraction of enzymes controlling the thickness, composition and consistency of the mucin glycans. Our data and analyses provide biochemical insights into the effects of antibiotic usage on the dynamics of the GIT microbiota and pin-point presumptive links to obesity. The knowledge and the hypotheses generated herein lay a foundation for subsequent, systematic research that will be paramount for the design of “smart” dietary and therapeutic interventions to modulate host-microbe metabolic co-regulation in intestinal homeostasis.	es_ES
dc.subject	antibiotic therapy	es_ES
dc.subject	distal gut	es_ES
dc.subject	glycosidase	es_ES
dc.subject	metabolic reconstruction	es_ES
dc.subject	obesity	es_ES
dc.title	Functional consequences of microbial shifts in the human gastrointestinal tract linked to antibiotic treatment and obesity	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.4161/gmic.25321	es_ES
dc.identifier.idgrec	090681	es_ES