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An Enriched European Eel Transcriptome Sheds Light upon Host-Pathogen Interactions with Vibrio vulnificus

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An Enriched European Eel Transcriptome Sheds Light upon Host-Pathogen Interactions with Vibrio vulnificus

dc.contributor.author	Callol Junyer, Agnès
dc.contributor.author	Reyes-López, Felipe E.
dc.contributor.author	Roig Molina, Francisco José
dc.contributor.author	Goetz, Giles
dc.contributor.author	Goetz, Frederick W.
dc.contributor.author	Amaro González, Carmen
dc.contributor.author	MacKenzie, Simon
dc.date.accessioned	2016-07-04T15:50:26Z
dc.date.available	2016-07-04T15:50:26Z
dc.date.issued	2015
dc.identifier.citation	Callol Junyer, Agnès Reyes-López, Felipe E. Roig Molina, Francisco José Goetz, Giles Goetz, Frederick W. Amaro González, Carmen MacKenzie, Simon 2015 An Enriched European Eel Transcriptome Sheds Light upon Host-Pathogen Interactions with Vibrio vulnificus Plos One 10 7
dc.identifier.uri	http://hdl.handle.net/10550/54360
dc.description.abstract	Infectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen-associated molecular patterns (PAMPs), extracted RNA from the immune-related tissues and sequenced the transcriptome. We obtained more than 2 x 10(6) reads that were assembled and annotated into 45,067 new descriptions with a notable representation of novel transcripts related with pathogen recognition, signal transduction and the immune response. Then, we designed a DNA-microarray that was used to analyze the early immune response against Vibrio vulnificus, a septicemic pathogen that uses the gills as the portal of entry into the blood, as well as the role of the main toxin of this species (RtxA13) on this early interaction. The gill transcriptomic profiles obtained after bath infecting eels with the wild type strain or with a mutant deficient in rtxA13 were analyzed and compared. Results demonstrate that eels react rapidly and locally against the pathogen and that this immune-response is rtxA13-dependent as transcripts related with cell destruction were highly up-regulated only in the gills from eels infected with the wild-type strain. Furthermore, significant differences in the immune response against the wild type and the mutant strain also suggest that host survival after V. vulnificus infection could depend on an efficient local phagocytic activity. Finally, we also found evidence of the presence of an interbranchial lymphoid tissue in European eel gills although further experiments will be necessary to identify such tissue.
dc.language.iso	eng
dc.relation.ispartof	Plos One, 2015, vol. 10, num. 7
dc.subject	Bacteris patògens
dc.subject	Microbiologia
dc.title	An Enriched European Eel Transcriptome Sheds Light upon Host-Pathogen Interactions with Vibrio vulnificus
dc.type	journal article	es_ES
dc.date.updated	2016-07-04T15:50:26Z
dc.identifier.doi	10.1371/journal.pone.0133328
dc.identifier.idgrec	107509
dc.rights.accessRights	open access	es_ES