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Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM)

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Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM)

dc.contributor.author	Tamborero Capilla, Silvia
dc.contributor.author	Vilar, Marçal
dc.contributor.author	Martínez Gil, Luis
dc.contributor.author	Johnson, Arthur E.
dc.contributor.author	Mingarro Muñoz, Ismael
dc.date.accessioned	2016-11-29T15:40:02Z
dc.date.available	2016-11-29T15:40:02Z
dc.date.issued	2011
dc.identifier.citation	Tamborero Capilla, Silvia Vilar, Marçal Martínez Gil, Luis Johnson, Arthur E. Mingarro Muñoz, Ismael 2011 Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM) Journal of Molecular Biology 406 4 571 582
dc.identifier.uri	http://hdl.handle.net/10550/56203
dc.description.abstract	The translocating chain-associating membrane protein (TRAM) is a glycoprotein involved in the translocation of secreted proteins into the endoplasmic reticulum (ER) lumen and in the insertion of integral membrane proteins into the lipid bilayer. As a major step toward elucidating the structure of the functional ER translocation/insertion machinery, we have characterized the membrane integration mechanism and the transmembrane topology of TRAM using two approaches: photocross-linking and truncated C-terminal reporter tag fusions. Our data indicate that TRAM is recognized by the signal recognition particle and translocon components, and suggest a membrane topology with eight transmembrane segments, including several poorly hydrophobic segments. Furthermore, we studied the membrane insertion capacity of these poorly hydrophobic segments into the ER membrane by themselves. Finally, we confirmed the main features of the proposed membrane topology in mammalian cells expressing full-length TRAM.
dc.language.iso	eng
dc.relation.ispartof	Journal of Molecular Biology, 2011, vol. 406, num. 4, p. 571-582
dc.subject	Proteïnes de membrana
dc.subject	Membranes (Biologia)
dc.title	Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM)
dc.type	journal article	es_ES
dc.date.updated	2016-11-29T15:40:03Z
dc.identifier.doi	10.1016/j.jmb.2011.01.009
dc.identifier.idgrec	067268
dc.rights.accessRights	open access	es_ES