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Sec61alpha and TRAM are Sequentially Adjacent to a Nascent Viral Membrane Protein during its ER Integration

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Sec61alpha and TRAM are Sequentially Adjacent to a Nascent Viral Membrane Protein during its ER Integration

dc.contributor.author	Saurí Peris, Ana
dc.contributor.author	McCormick, Peter J.
dc.contributor.author	Johnson, Arthur E.
dc.contributor.author	Mingarro Muñoz, Ismael
dc.date.accessioned	2016-11-29T18:24:13Z
dc.date.available	2016-11-29T18:24:13Z
dc.date.issued	2007
dc.identifier.citation	Saurí Peris, Ana McCormick, Peter J. Johnson, Arthur E. Mingarro Muñoz, Ismael 2007 Sec61alpha and TRAM are Sequentially Adjacent to a Nascent Viral Membrane Protein during its ER Integration Journal of Molecular Biology 366 2 366 374
dc.identifier.uri	http://hdl.handle.net/10550/56207
dc.description.abstract	Co-translational integration of a nascent viral membrane protein into the endoplasmic reticulum membrane takes place via the translocon. We have been studying the early stages of the integration of a double-spanning plant viral movement protein to gain insights into how viral membrane proteins are transferred from the hydrophilic interior of the translocon into the hydrophobic environment of the bilayer, where the transmembrane (TM) segments of the viral proteins can diffuse freely. Photocrosslinking experiments reveal that this integration involves the sequential passage of the TM segments past Sec61alpha and translocating chain-associating membrane protein (TRAM). Each TM segment is first adjacent to Sec61alpha and subsequently is adjacent to TRAM. TRAM crosslinking extends for a long period during nascent chain biogenesis. In addition, the replacement of the first viral TM segment with a non-viral TM sequence still yields nascent chain photo-adducts with TRAM. TRAM therefore appears to be involved in viral membrane protein integration, and nascent chain recognition by TRAM does not appear to rely solely on the TM domains.
dc.language.iso	eng
dc.relation.ispartof	Journal of Molecular Biology, 2007, vol. 366, num. 2, p. 366-374
dc.subject	Proteïnes de membrana
dc.subject	Membranes (Biologia)
dc.title	Sec61alpha and TRAM are Sequentially Adjacent to a Nascent Viral Membrane Protein during its ER Integration
dc.type	journal article	es_ES
dc.date.updated	2016-11-29T18:24:14Z
dc.identifier.doi	10.1016/j.jmb.2006.11.052
dc.identifier.idgrec	039202
dc.rights.accessRights	open access	es_ES