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Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy

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Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy

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dc.contributor.author Navarro Escobar, Esther es
dc.contributor.author González Rodríguez, Mª Paloma es
dc.contributor.author Ferrer Luque, Carmen María es
dc.date.accessioned 2017-07-27T08:47:08Z
dc.date.available 2017-07-27T08:47:08Z
dc.date.issued 2010 es
dc.identifier.citation Navarro Escobar, Esther ; González Rodríguez, Mª Paloma ; Ferrer Luque, Carmen María. Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 15 1 2010: 20- es
dc.identifier.uri http://hdl.handle.net/10550/60405
dc.description.abstract Aim: To evaluate the cytotoxicity of 15% citric acid, 5% phosphoric acid and 2.5% NaOCl on cultured fibroblasts using MTT colorimetric assay. Methodology: Irrigating solutions of 5% phosphoric acid, 15% citric acid, and 2.5% NaOCl, diluted at 0.1% and 0.5%, were applied to cell cultures of 3T3L1 fibroblasts. The cell viability was determined by means of MTT colorimetric assay after a period of 1, 6 and 24 hours. Percentages of cell viability were analyzed using the Kruskal-Wallis test for global comparisons and the Mann-Whitney U-test for pairwise comparisons. Results: The percentage of cell viability diminished progressively over a 24 hour period in all solutions at both dilutions. At 0.1% dilution, 2.5% NaOCl (63.39%) and 15% citric acid (53.91%) showed the highest percentage of cell viability (p=0.083). At 0.5% dilution, 2.5% NaOCl again showed the highest cell viability value (48.51%). Conclusions: The irrigating solution with the highest percentage of cell viability was 2.5% NaOCl at both 0.1% and 0.5% dilutions. A very low percentage of cell viability was obtained with 15% citric acid and 5% phosphoric acid at 0.5% dilution. es
dc.title Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.15.e90 es
dc.type.hasVersion VoR es_ES

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