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Stable and simple quantitative phase-contrast imaging by Fresnel biprism

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Stable and simple quantitative phase-contrast imaging by Fresnel biprism

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dc.contributor.author	Ebrahimi, Samira
dc.contributor.author	Dashtdar, Masoomeh
dc.contributor.author	Sánchez Ortiga, Emilio
dc.contributor.author	Martínez Corral, Manuel
dc.contributor.author	Javidi, Bahram
dc.date.accessioned	2018-04-13T13:34:15Z
dc.date.available	2018-04-13T13:34:15Z
dc.date.issued	2018
dc.identifier.citation	Ebrahimi, Samira Dashtdar, Masoomeh Sánchez Ortiga, Emilio Martínez Corral, Manuel Javidi, Bahram 2018 Stable and simple quantitative phase-contrast imaging by Fresnel biprism Applied Physics Letters 112 11 113701
dc.identifier.uri	http://hdl.handle.net/10550/65659
dc.description.abstract	Digital holographic (DH) microscopy has grown into a powerful nondestructive technique for the real-time study of living cells including dynamic membrane changes and cell fluctuations in nanometer and sub-nanometer scales. The conventional DH microscopy configurations require a separately generated coherent reference wave that results in a low phase stability and a necessity to precisely adjust the intensity ratio between two overlapping beams. In this work, we present a compact, simple, and very stable common-path DH microscope, employing a self-referencing configuration. The microscope is implemented by a diode laser as the source and a Fresnel biprism for splitting and recombining the beams simultaneously. In the overlapping area, linear interference fringes with high contrast are produced. The frequency of the interference pattern could be easily adjusted by displacement of the biprism along the optical axis without a decrease in fringe contrast. To evaluate the validity of the method, the spatial noise and temporal stability of the setup are compared with the common off-axis DH microscope based on a Mach-Zehnder interferometer. It is shown that the proposed technique has low mechanical noise as well as superb temporal stability with sub-nanometer precision without any external vibration isolation. The higher temporal stability improves the capabilities of the microscope for studying micro-object fluctuations, particularly in the case of biological specimens. Experimental results are presented using red blood cells and silica microspheres to demonstrate the system performance.
dc.language.iso	eng
dc.relation.ispartof	Applied Physics Letters, 2018, vol. 112, num. 11, p. 113701
dc.subject	Microscòpia
dc.subject	Òptica
dc.title	Stable and simple quantitative phase-contrast imaging by Fresnel biprism
dc.type	journal article	es_ES
dc.date.updated	2018-04-13T13:34:16Z
dc.identifier.doi	10.1063/1.5021008
dc.identifier.idgrec	124410
dc.rights.accessRights	open access	es_ES