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An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing

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An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing

dc.contributor.author	Montero Pau, Javier
dc.contributor.author	Blanca, José
dc.contributor.author	Esteras, Cristina
dc.contributor.author	Martínez-Pérez, Eva M.
dc.contributor.author	Gómez, Pedro
dc.contributor.author	Monforte, Antonio J.
dc.contributor.author	Cañizares, Joaquín
dc.contributor.author	Picó, Belén
dc.date.accessioned	2018-11-05T15:09:43Z
dc.date.available	2018-11-05T15:09:43Z
dc.date.issued	2017
dc.identifier.citation	Montero Pau, Javier Blanca, José Esteras, Cristina Martínez-Pérez, Eva M. Gómez, Pedro Monforte, Antonio J. Cañizares, Joaquín Picó, Belén 2017 An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing Bmc Genomics 18 94 1 21
dc.identifier.uri	http://hdl.handle.net/10550/67855
dc.description.abstract	Background Cucurbita pepo is a cucurbit with growing economic importance worldwide. Zucchini morphotype is the most important within this highly variable species. Recently, transcriptome and Simple Sequence Repeat (SSR)- and Single Nucleotide Polymorphism (SNP)-based medium density maps have been reported, however further genomic tools are needed for efficient molecular breeding in the species. Our objective is to combine currently available complete transcriptomes and the Zucchini genome sequence with high throughput genotyping methods, mapping population development and extensive phenotyping to facilitate the advance of genomic research in this species. Results We report the Genotyping-by-sequencing analysis of a RIL population developed from the inter subspecific cross Zucchini x Scallop (ssp. pepo x ssp. ovifera). Several thousands of SNP markers were identified and genotyped, followed by the construction of a high-density linkage map based on 7,718 SNPs (average of 386 markers/linkage group) covering 2,817.6 cM of the whole genome, which is a great improvement with respect to previous maps. A QTL analysis was performed using phenotypic data obtained from the RIL population from three environments. In total, 48 consistent QTLs for vine, flowering and fruit quality traits were detected on the basis of a multiple-environment analysis, distributed in 33 independent positions in 15 LGs, and each QTL explained 1.5-62.9% of the phenotypic variance. Eight major QTLs, which could explain greater than 20% of the phenotypic variation were detected and the underlying candidate genes identified. Conclusions Here we report the first SNP saturated map in the species, anchored to the physical map. Additionally, several consistent QTLs related to early flowering, fruit shape and length, and rind and flesh color are reported as well as candidate genes for them. This information will enhance molecular breeding in C. pepo and will assist the gene cloning underlying the studied QTLs, helping to reveal the genetic basis of the studied processes in squash.
dc.language.iso	eng
dc.relation.ispartof	Bmc Genomics, 2017, vol. 18, num. 94, p. 1-21
dc.subject	Genòmica
dc.subject	Gens Mapatge
dc.title	An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing
dc.type	journal article	es_ES
dc.date.updated	2018-11-05T15:09:44Z
dc.identifier.doi	10.1186/s12864-016-3439-y
dc.identifier.idgrec	127100
dc.rights.accessRights	open access	es_ES