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Low-fouling, mixed-charge poly-L-lysine polymers with anionic oligopeptide side-chains

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Low-fouling, mixed-charge poly-L-lysine polymers with anionic oligopeptide side-chains

dc.contributor.author	Bellassai, Noemi
dc.contributor.author	Martí Morant, Almudena
dc.contributor.author	Spoto, Giuseppe (químic), 1955-
dc.contributor.author	Huskens, Jurriaan
dc.date.accessioned	2019-01-10T15:21:48Z
dc.date.available	2019-01-10T15:21:48Z
dc.date.issued	2018
dc.identifier.citation	Bellassai, Noemi; Martí Morant, Almudena; Spoto, Giuseppe; Huskens, Jurriaan (2018). Low-fouling, mixed-charge poly-L-lysine polymers with anionic oligopeptide side-chains. Journal Of Materials Chemistry B 6, 46, 7662-7673
dc.identifier.uri	http://hdl.handle.net/10550/68441
dc.description.abstract	Biosensors and biomedical devices require antifouling surfaces to prevent the non-specific adhesion of proteins or cells, for example, when aiming to detect circulating cancer biomarkers in complex natural media (e.g., in blood plasma or serum). A mixed-charge polymer was prepared by the coupling of a cationic polyelectrolyte and an anionic oligopeptide through a modified 'grafting-to' method. The poly-L-lysine (PLL) backbone was modified with different percentages (y%) of maleimide-NHS ester chains (PLL-mal(y%), from 13% to 26%), to produce cationic polymers with specific grafting densities, obtaining a mixed-charge polymer. The anionic oligopeptide structure (CEEEEE) included one cysteine (C) and five glutamic acid (E) units, which were attached to the PLL-mal(y%) polymers, preadsorbed on gold substrates, through the thiol-maleimide Michael-type addition. Contact angle and PM-IRRAS data confirmed monolayer formation of the modified PLLs. Antifouling properties of peptide-PLL surfaces were assessed in adsorption studies using quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance imaging (SPRI) techniques. PLL-mal(26%)-CEEEEE showed the best antifouling performance in single-protein solutions, and the nonspecific adsorption of proteins was 46 ng cm−2 using diluted human plasma samples. The new PLL-mal(26%)-CEEEEE polymer offers a prominent low-fouling activity in complex media, with rapid and simple procedures for the synthesis and functionalization of the surface compared to conventional non-fouling materials.
dc.language.iso	eng
dc.relation.ispartof	Journal Of Materials Chemistry B, 2018, vol. 6, num. 46, p. 7662-7673
dc.subject	Química
dc.subject	Materials nanoestructurats
dc.title	Low-fouling, mixed-charge poly-L-lysine polymers with anionic oligopeptide side-chains
dc.type	journal article	es_ES
dc.date.updated	2019-01-10T15:21:48Z
dc.identifier.doi	10.1039/C8TB01619D
dc.identifier.idgrec	129298
dc.rights.accessRights	open access	es_ES