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Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions

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Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions

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dc.contributor.author	Navarro-Sánchez, José
dc.contributor.author	Almora-Barrios, Neyvis
dc.contributor.author	Lerma-Berlanga, Belén
dc.contributor.author	Ruiz Pernía, José Javier
dc.contributor.author	Lórenz Fonfría, Victor Armando
dc.contributor.author	Tuñón, Iñaki
dc.contributor.author	Martí Gastaldo, Carlos
dc.date.accessioned	2019-05-02T14:55:56Z
dc.date.available	2019-05-02T14:55:56Z
dc.date.issued	2019
dc.identifier.citation	Navarro-Sánchez, José Almora-Barrios, Neyvis Lerma-Berlanga, Belén Ruiz Pernía, José Javier Lórenz Fonfría, Victor Armando Tuñón, Iñaki Martí Gastaldo, Carlos 2019 Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions Chemical Science 10 4082 4088
dc.identifier.uri	http://hdl.handle.net/10550/70041
dc.description.abstract	Enzymatic catalysis is of great importance to the chemical industry. However, we are still scratching the surface of the potential of biocatalysis due to the limited operating range of enzymes in harsh environments or their low recyclability. The role of Metal-Organic Frameworks (MOFs) as active supports to help overcome these limitations, mainly by immobilization and stabilization of enzymes, is rapidly expanding. Here we make use of mild heating and a non-polar medium during incubation to induce the translocation of a small enzyme like protease in the mesoporous MOF MIL-101(Al)-NH2. Our proteolytic tests demonstrate that protease@MIL-101(Al)-NH2 displays higher activity than the free enzyme under all the conditions explored and, more importantly, its usability can be extended to extreme conditions of pH and high temperatures. MOF immobilization is also effective in providing the biocomposite with long-term stability, recyclability and excellent compatibility with competing enzymes. This simple, one-step infiltration strategy might accelerate the discovery of new MOF-enzyme biocatalysts that meet the requirements for biotechnological applications.
dc.language.iso	eng
dc.relation.ispartof	Chemical Science, 2019, vol. 10, p. 4082-4088
dc.subject	Química organometàl·lica
dc.title	Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions
dc.type	journal article	es_ES
dc.date.updated	2019-05-02T14:55:57Z
dc.identifier.doi	10.1039/C9SC00082H
dc.identifier.idgrec	131669
dc.rights.accessRights	open access	es_ES