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Nanosecond pulsed electric field inhibits malignant melanoma growth by inducing the change of systemic immunity

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Nanosecond pulsed electric field inhibits malignant melanoma growth by inducing the change of systemic immunity

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dc.contributor.author Zhang, Xingxing es
dc.contributor.author Zhang, Yunxia es
dc.contributor.author Chen, Jian es
dc.contributor.author Wu, Yuyan es
dc.contributor.author Zhang, Jue es
dc.contributor.author Wang, Jing es
dc.date.accessioned 2019-11-06T12:47:58Z
dc.date.available 2019-11-06T12:47:58Z
dc.date.issued 2019 es
dc.identifier.citation Zhang, Xingxing ; Zhang, Yunxia ; Chen, Jian ; Wu, Yuyan ; Zhang, Jue ; Wang, Jing. Nanosecond pulsed electric field inhibits malignant melanoma growth by inducing the change of systemic immunity. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 24 4 2019: 5- es
dc.identifier.uri https://hdl.handle.net/10550/72045
dc.description.abstract Nanosecond pulsed electric fields (nsPEFs) showed an inhibitory effect on proliferation of malignant melanoma. In this study, the growth of melanoma were inhibited by changing the systemic immunity. C57BL/6 mice with B16 malignant were exposed to 200 pulses of 100 ns duration, 30kV/cm. The mice were executed four days later. T lymphocyte has been extracted from spleen. Cell viability was evaluated by CCK-8 assay. CD3+CD4+ T cells, CD3+CD8+ T cells, regulatory T cells (Treg) and myeloid-derived suppressor cells (MDSC) were analyzed by flow cytometry. TNF-?, IL-2, IL-10, TGF-?, IFN? ? levels in supernatants were assessed by ELISA. C57 malignant melanoma model were established successfully. After the treatment of nsPEFs(30 kV/cm 100 ns 200p), the numbers of T lymphocytes were increased.CD3+ CD4+ T cells changed from 48% to 51.2%;CD3+CD8+T lymphocytes increased from 39.6% to 40.4%.Treg cells reduced from 4.3% to 2.4%,MDSC decreased by 39.0% to 19.7% . In addition, the level of TNF-?, IL-2 were increased (P< 0.05) and the level of IL-10 were decreased (P<0.05) and the level of TGF-? and IFN-? remained stable (P>0.05). Tumor growth can be effectively inhibited by nsPEFs in vivo, which activate targets of immune respones, accumulation of inflammatory cells and immune cytokines. es
dc.title Nanosecond pulsed electric field inhibits malignant melanoma growth by inducing the change of systemic immunity es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.22976 es
dc.type.hasVersion VoR es_ES

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