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Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)

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Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)

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dc.contributor.author Sánchez-Garcés, María Angeles es
dc.contributor.author Jorba, Marta es
dc.contributor.author Ciurana, Joan es
dc.contributor.author Viñas Ciordia, Miguel es
dc.contributor.author Vinuesa, Teresa es
dc.date.accessioned 2019-11-06T12:51:52Z
dc.date.available 2019-11-06T12:51:52Z
dc.date.issued 2019 es
dc.identifier.citation Sánchez-Garcés, María Angeles ; Jorba, Marta ; Ciurana, Joan ; Viñas Ciordia, Miguel ; Vinuesa, Teresa. Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety). En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 24 5 2019: 8- es
dc.identifier.uri https://hdl.handle.net/10550/72066
dc.description.abstract Background: The reuse of implant healing abutments is common in dental practice. Effective elimination of bacteria and viruses is accomplished by conventional sterilization. The aim of this work was to explore the eventual survival of microorganisms on sterilized healing abutments and to rule out the presence of transmissible organic material after standard procedures. Material and Methods: A total of 55 healing abutments previously used in patients will be washed and sterilized in a steam autoclave at 121ºC for 15 min. Each healing abutment will be cultured in Brain Heart Infusion broth (BHI) under strict aseptic conditions. Besides, two control groups will be included: one of 3 unused healing abutments, and the other of just medium. After 10 days at 37°C under a 5% CO2 100 µl of the broth will be plated on solid media (Brain Infusion Agar, BHIA) and Columbia Blood agar to test for sterility. The remaining volume will be centrifuged, the sediment fixed, and a Gram stain performed to discard the presence of non-cultivable microorganisms. Moreover, to determine the presence of remaining organic material after the cleaning and sterilizing treatments, the bioburden will be determined by measuring total organic carbon (TOC) in another 10 previously used healing abutments, cleaned and sterilized, that will be submerged in Milli-Q water and sonicated. Results: No bacterial growth was detected on any of the 58 cultured abutments, indicating that the sterilization was completely satisfactory in terms of removal of live bacteria or spores. Nevertheless, significant amounts of organic carbon may still be recovered (up to 125,31 µg/abutment) after they have been sterilized. Conclusions: Significant amounts of the bioburden remained adhered to the surfaces in spite of the cleaning and sterilization procedures. Taking into account our results and data from other authors, the presence of infectious particles on the reused healing abutments such as prions cannot be ruled out. es
dc.title Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety) es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.22967 es
dc.type.hasVersion VoR es_ES

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