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Different methods of cell quantification can lead to different results : a comparison of digital methods using a pilot study of dendritic cells in HIV-positive patients

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Different methods of cell quantification can lead to different results : a comparison of digital methods using a pilot study of dendritic cells in HIV-positive patients

dc.contributor.author	Fernandes, Diego-Tetzner	es
dc.contributor.author	van Heerden, Willie F.P.	es
dc.contributor.author	Ribeiro, Ana-Carolina-Prado	es
dc.contributor.author	Brandao, Thais-Bianca	es
dc.contributor.author	de Mello, Evandro Sobroza	es
dc.contributor.author	Rivera, Cesar A.	es
dc.contributor.author	van Heerden, Marlene B.	es
dc.contributor.author	Gondak, Rogério Oliveira	es
dc.contributor.author	Santos Silva, Alan Roger	es
dc.contributor.author	Vargas, Pablo Agustín	es
dc.contributor.author	Lopes, Márcio Ajudarte	es
dc.date.accessioned	2021-01-15T09:07:36Z
dc.date.available	2021-01-15T09:07:36Z
dc.date.issued	2020	es
dc.identifier.citation	Fernandes, Diego-Tetzner ; van Heerden, Willie F.P. ; Ribeiro, Ana-Carolina-Prado ; Brandao, Thais-Bianca ; de Mello, Evandro Sobroza ; Rivera, Cesar A. ; van Heerden, Marlene B. ; Gondak, Rogério Oliveira ; Santos Silva, Alan Roger ; Vargas, Pablo Agustín ; Lopes, Márcio Ajudarte. Different methods of cell quantification can lead to different results : a comparison of digital methods using a pilot study of dendritic cells in HIV-positive patients. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 25 3 2020: 3-	es
dc.identifier.uri	https://hdl.handle.net/10550/77126
dc.description.abstract	Although new digital pathology tools have improved the positive cell quantification, there is a heterogeneity of the quantification methods in the literature. The aim of this study was to evaluate and propose a novel dendritic cells quantification method in squamous cell carcinoma comparing it with a conventional quantification method. Twenty-six squamous cell carcinomas HIV-positive cases affecting the oropharynx, lips and oral cavity were selected. Immunohistochemistry for CD1a, CD83, and CD207 was performed. The immunohistochemical stains were evaluated by automated examination using a positive pixel count algorithm. A conventional quantification method (unspecific area method; UA) and a novel method (specific area method; SA) were performed obtaining the corresponding density of positive dendritic cells for the intratumoral and peritumoral regions. The Mann-Whitney U test was used to verify the influence of the quantification methods on the positive cell counting according to the evaluated regions. Data were subjected to the ANOVA and Student?s t-test to verify the influence of the tumour location, stage, histological grade, and amount of inflammation on the dendritic cells density counting. The cell quantification method affected the dendritic cells counting independently of the evaluated region (P-value <0.05). Significant differences between methods were also observed according to the tumour features evaluations. The positive cell quantification method influences the dendritic cells density results. Unlike the conventional method (UA method), the novel SA method avoids non-target areas included in the hotspots improving the reliability and reproducibility of the density cell quantification.	es
dc.title	Different methods of cell quantification can lead to different results : a comparison of digital methods using a pilot study of dendritic cells in HIV-positive patients	es
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	es
dc.identifier.doi	10.4317/medoral.23472	es
dc.type.hasVersion	VoR	es_ES