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Evaluation of two different concentration methods for surveillance of human viruses in sewage and their effects on SARS-CoV-2 sequencing

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Evaluation of two different concentration methods for surveillance of human viruses in sewage and their effects on SARS-CoV-2 sequencing

dc.contributor.author	Girón-Guzmán, Iris Paola
dc.contributor.author	Díaz Reolid, Azahara
dc.contributor.author	Cuevas Ferrando, Enric
dc.contributor.author	Comas Espadas, Iñaki
dc.contributor.author	Pérez Cataluña, Alba
dc.contributor.author	Sánchez, Gloria
dc.contributor.author	Falcó Ferrando, Irene Lourdes
dc.date.accessioned	2023-05-24T14:27:58Z
dc.date.available	2023-05-24T14:27:58Z
dc.date.issued	2022
dc.identifier.citation	Girón-Guzmán, Iris Paola Díaz Reolid, Azahara Cuevas Ferrando, Enric Comas Espadas, Iñaki Pérez Cataluña, Alba Sánchez, Gloria Falcó Ferrando, Irene Lourdes 2022 Evaluation of two different concentration methods for surveillance of human viruses in sewage and their effects on SARS-CoV-2 sequencing Science of the Total Environment 862 160914
dc.identifier.uri	https://hdl.handle.net/10550/86908
dc.description.abstract	During the current COVID-19 pandemic, wastewater-based epidemiology (WBE) emerged as a reliable strategy both as a surveillance method and a way to provide an overview of the SARS-CoV-2 variants circulating among the population. Our objective was to compare two different concentration methods, a well-established aluminum-based procedure (AP) and the commercially available Maxwell® RSC Enviro Wastewater TNA Kit (TNA) for human enteric virus, viral indicators and SARS-CoV-2 surveillance. Additionally, both concentration methods were analyzed for their impact on viral infectivity, and nucleic acids obtained from each method were also evaluated by massive sequencing for SARS-CoV-2. The percentage of SARS-CoV-2 positive samples using the AP method accounted to 100 %, 83.3 %, and 33.3 % depending on the target region while 100 % positivity for these same three target regions was reported using the TNA procedure. The concentrations of norovirus GI, norovirus GII and HEV using the TNA method were significantly greater than for the AP method while no differences were reported for rotavirus, astrovirus, crAssphage and PMMoV. Furthermore, TNA kit in combination with the Artic v4 primer scheme yields the best SARS-CoV-2 sequencing results. Regarding impact on infectivity, the concentration method used by the TNA kit showed near-complete lysis of viruses. Our results suggest that although the performance of the TNA kit was higher than that of the aluminum procedure, both methods are suitable for the analysis of enveloped and non-enveloped viruses in wastewater by molecular methods.
dc.language.iso	eng
dc.relation.ispartof	Science of the Total Environment, 2022, vol. 862, p. 160914
dc.subject	Epidemiologia
dc.title	Evaluation of two different concentration methods for surveillance of human viruses in sewage and their effects on SARS-CoV-2 sequencing
dc.type	journal article
dc.date.updated	2023-05-24T14:27:58Z
dc.identifier.doi	10.1016/j.scitotenv.2022.160914
dc.identifier.idgrec	159259
dc.rights.accessRights	open access