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Candida albicans UBI3 and UBI4 promoter regions confer differential regulation of invertase production to Saccharomyces cerevisiae cells in response to stress

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Candida albicans UBI3 and UBI4 promoter regions confer differential regulation of invertase production to Saccharomyces cerevisiae cells in response to stress

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dc.contributor.author Roig Montoya, Patricia
dc.contributor.author Gozalbo Flor, Daniel
dc.date.accessioned 2010-06-14T08:19:58Z
dc.date.available 2010-06-14T08:19:58Z
dc.date.issued 2002
dc.identifier.citation ROIG P. ; Gozalbo Flor, Daniel, 2002, Candida albicans UBI3 and UBI4 promoter regions confer differential regulation of invertase production to Saccharomyces cerevisiae cells in response to stress, International Microbiology, vol. 5, no. 1, p. 33–36 en
dc.identifier.uri http://hdl.handle.net/10550/12957
dc.description.abstract Candida albicans ubiquitin genes UBI3 and UBI4 encode a ubiquitin-hybrid protein involved in ribosome biogenesis and polyubiquitin, respectively. In this work we show that UBI3 and UBI4 promoter regions confer differentialexpr ession consistent with the function of their encoded gene products. Hybrid genes were constructed containing the SUC2 coding region under the controlof UBI3 or UBI4 promoters in the yeast vector pLC7. Invertase production in Saccharomyces cerevisiae transformants was differentially regulated: the UBI4 promoter was induced by stress conditions (thermalupshift and/or starvation) whereas the UBI3 promoter conferred constitutive invertase production in growing yeast cells. These results indicate that the UBI4 promoter is regulated by stress-response signaling pathways, whereas the UBI3 promoter is controlled according to the requirement for protein synthesis to support cell growth. en
dc.language.iso es en
dc.subject Candida albicans ; Ubiquitin genes ; Invertase ; Saccharomyces cerevisiae ; Promoter gene fusion ; Heterologous expression en
dc.title Candida albicans UBI3 and UBI4 promoter regions confer differential regulation of invertase production to Saccharomyces cerevisiae cells in response to stress en
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA en
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Microbiología en
dc.identifier.doi 10.1007/s10123-002-0056-1 en
dc.identifier.idgrec 031108 en
dc.type.hasVersion VoR es_ES

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