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Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH

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Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH

dc.contributor.author	Zaragozá Colom, Rosa
dc.contributor.author	Miralles Fernández, Vicente
dc.contributor.author	Rus, Diana
dc.contributor.author	García de Mier, Concepción
dc.contributor.author	Carmena Rodríguez, Rafael
dc.contributor.author	Ruíz García-Trevijano, Elena
dc.contributor.author	Barber Sanchis, Teresa
dc.contributor.author	Pallardó, Federico V.
dc.contributor.author	Torres Asensi, Luis
dc.contributor.author	Viña Ribes, Juan
dc.date.accessioned	2010-06-29T10:34:34Z
dc.date.available	2010-06-29T10:34:34Z
dc.date.issued	2005
dc.identifier.citation	Zaragozá, R.; Miralles, J.V.; Rus, A.D.; García, C.; Carmena, R.; García-Trevijano, E.R.; Barber, T.; Pallardó, F.V.; Torres, L.; Viña, J.R. (2005) Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH. En Biochemical Journal. 391, 581–588	en
dc.identifier.uri	http://hdl.handle.net/10550/13993
dc.description.abstract	Zaragozá, R; Miralles, VJ; Rus, AD; García, C; Carmena, R; García-Trevijano, ER; Barber, T; Pallardó, FV; Torres, L; Viña, JR. At the end of lactation the mammary gland undergoes involution, a process characterized by apoptosis of secretory cells and tissue remodelling. To gain insight into this process, we analysed the gene expression profile by oligonucleotide microarrays during lactation and after forced weaning. Up-regulation of inflammatory mediators and acute-phase response genes during weaning was found. Expression of IκBα (inhibitory κBα), a protein known to modulate NF-κB (nuclear factor-κB) nuclear translocation, was significantly up-regulated. On the other hand, there was a time-dependent degradation of IκBα protein levels in response to weaning, suggesting a role for NF-κB. Furthermore, we have demonstrated, using chromatin immunoprecipitation assays, binding of NF-κB to the NOS-2 (inducible nitric oxide synthase) promoter at the early onset of events triggered during weaning. The three isoforms of NOS are constitutively present in the lactating mammary gland; however, while NOS-2 mRNA and protein levels and, consequently, NO production are increased during weaning, NOS-3 protein levels are diminished. Western blot analyses have demonstrated that protein nitration is increased in the mammary gland during weaning, but this is limited to a few specific tyrosine-nitrated proteins. Interestingly, inhibition of GSH synthesis at the peak of lactation partially mimics these findings, highlighting the role of NO production and GSH depletion during involution.	en
dc.language.iso	en	en
dc.subject	GSH	en
dc.subject	involution	en
dc.subject	lactation	en
dc.subject	nitric oxide	en
dc.subject	NF-κB	en
dc.title	Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH	en
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS DE LA VIDA::Bioquímica	en
dc.identifier.doi	10.1042/BJ20050507	en
dc.identifier.idgrec	023286	en
dc.type.hasVersion	VoR	es_ES
dc.identifier.url	http://www.biochemj.org/bj/391/0581/3910581.pdf	en