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dc.contributor.advisor | Lloris Carsí, José Miguel | es_ES |
dc.contributor.advisor | Cejalbo Lapeña, Dolores | es_ES |
dc.contributor.author | Chanzá-Aviñó, Mercedes | es_ES |
dc.contributor.other | Universitat de València - CIRURGIA | es_ES |
dc.date.accessioned | 2010-07-07T08:24:54Z | |
dc.date.available | 2010-07-07T08:24:54Z | |
dc.date.issued | 1996 | es_ES |
dc.date.submitted | 1996-05-24 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10550/15551 | |
dc.description.abstract | RESUMEN Se realizó un estudio experimental con ratas macho cepa Wistar, para valorar el efecto de la Pentoxifilina (PTX) en la lesión por Isquemia-Repefusión intestinal y de esta forma considerar la conveniencia o no de su utilización, en el caso de trasplante de Intestino Delgado. Material y métodos Se distribuyeron aleatoriamente 150 ratas en 6 grupos: 2 controles, alos que se administró suero fisiológico, y 4 grupos con tratamiento Pentoxifilina: grupo Pl (1 mg/Kg de PTX 1 hora antes de isquemia), grupo P2 (1 mg/Kg de PTX en el momento de la reperfusión), grupo P3 (10 mg/ Kg de PTX 1 hora antes de la isquemia), grupo P4 (10 mg/Kg de PT'X en momento de la reperfusión). La isquemia intestinal se realizó con clampaje de la arteria mesentérica superior, durante 120 minutos, siendo el tiempo de reperfusión empleado de 1 hora. El fármaco ensayado fue la Pentoxifilina, que es un agente hemorreologico de la familia de las metibcantinas. Sus propiedades más destacadas son el aumento de la deformabilidad eritrocitaria, la disminución de la viscosidad sanguínea y el aumento de la capacidad fibrinolítica del plasma, efectos que contribuyen al aumento del flujo circulatorio. Determinaciones realizadas: - Estudio de supervivencia a17° día de la intervención. - Pruebas Bioquímicas: Urea, Creatinina, Sodio, Potasio, Calcio, Clo ro, Fósforo, LDH. - Determinaciones de Malonildialdehído y Mieloperoxidasa en el tjido intestinal isquémico, que nos valoran el grado de peroxidación lipídica y de infiltración neutrofílica, respectivamente. - Estudio anatomopatológico de los cortes intestinales. Estudio de la microcirculación intestinal mediante la técnica del Láser Doppler, recogiendo las mediciones ininterrumpidamente desde antes de la isquemia hasta 1 hora después de la reperfusión. Conclusiones Los resultados obtenidos en el presente estudio sugieren que la Pentoxifilina: 1. Influye favorablemente en la lesión por isquemia-reperfusión intestinal. 2. Protege la circulación intestinal, gracias a sus acciones hemorreológicas. 3. Minimiza el proceso de peroxidación lipídica. 4. El efecto protector de la PTX se muestra dependiente del momento de administración, siendo óptimo el tiempo cero de la reperfusión. 5. Su acción no es dosis /dependiente en el rango de tratamiento utilizado. __________________________________________________________________________________________________ | es_ES |
dc.description.abstract | A study was carried out using male Wistar rats to assess the effect of Pentoxifilina (PTX) on the intestinal lesion caused by Ischemia-Reperfusion and thus consider how suitable its use is in cases of small intestine transplant. Material and method One hundred and fifty rats were placed randomly into 6 groups awo control g4:oups, which were given physiological serum (saline solution?) and 4 groups wffich were treated with Pentoxifilina. Group P1 (1 mg/kg of PTX one hour before is'chérhia), group P2 (1 mg/kg of PTX at the moment of reperfusion), group P3 (10/kg of PTX 1 hour before ischemia), groupP4 (10mg/kg of PTX at the moment of reperfusion). The intestinal ischer,nia y,ras done by clamping the superior mesenteric artery for 120 minutes, the timé nécessary for 1 hour reperfusion. The drug used was Pentoxifilina, which is a haemorrhaging agent belonging to the mefn1ipa flámily. Its most notable properties are the increase in eritrocitary deformation decrease in blood viscosity and the increase in the fibrinolitical ability of plasma, effects which contribute to the increase in blood circulation. Tests carried out.: Study of the survival rate at 17 days Biochemical analyses :urea, creatinin, sodium, potassium, calcium, chlorine, phosphorus, LDH. Analyses of malonildialdehyde and mieloperoxidasa in the ischemic intestinal tissue, which gives us the degree of lipid peroxidation and neutrophylic infiltration, respectively. Anatomopathological study of intestinal cuts. Study of intestinal microcirculation using the Laser Doppler technique, reading the measurements without interruption since one hour before the ischemia until 1 hour after the reperfusion. Conclusions The results from this study suggest that Pentoxifilina 1.has a favourable outcome on the lesion caused by intestinal reperfusion 2. protects intestinal circulation thanks to it haemorrhaging action 3. minimizes the lipid peroxidation process 4. the protective effect of PU depends on the moment of its administration, the best timé being rrioment zero of feperfusipn 5 Its efifect 15 nbt dependent on dose in the range of treatment used THE EFFECT OF PENTOXIFILINA TREATMENT ON INTESTINAL ISCHEMIA.REPERFUSION A study was carried out using male Wistar rats to assess the effect of Pentoxifilina (PTX) on the intestinal lesion caused by Ischemia-Reperfusion and thus consider how suitable its use is in cases of small intestine transplant. Material and method One hundred and fifty rats were placed randomly into 6 groups awo control g4:oups, which were given physiological serum (saline solution?) and 4 groups wffich were treated with Pentoxifilina. Group P1 (1 mg/kg of PTX one hour before is'chérhia), group P2 (1 mg/kg of PTX at the moment of reperfusion), group P3 (10/kg of PTX 1 hour before ischemia), groupP4 (10mg/kg of PTX at the moment of reperfusion). The intestinal ischer,nia y,ras done by clamping the superior mesenteric artery for 120 minutes, the timé nécessary for 1 hour reperfusion. The drug used was Pentoxifilina, which is a haemorrhaging agent belonging to the metibcantina flámily. Its most notable properties are the increase in eritrocitary deformation the decrease in blood viscosity and the increase in the fibrinolitical ability of plasma, effects which contribute to the increase in blood circulation. Tests carried out.: Study of the survival rate at 17 days Biochemical analyses :urea, creatinin, sodium, potassium, calcium, chlorine, phosphorus, LDH . Analyses of malonildialdehyde and mieloperoxidasa in the ischemic intestinal tissue, which gives us the degree of lipid peroxidation and neutrophylic infiltration, respectively. Anatomopathological study of intestinal cuts. Study of intestinal microcirculation using the Laser Doppler technique, reading the measurements without interruption since one hour before the ischemia until 1 hour after the reperfusion. Conclusions The results from this study suggest that Pentoxifilina 1.has a favourable outcome on the lesion caused by intestinal reperfusion 2. protects intestinal circulation thanks to it haemorrhaging action 3. minimizes the lipid peroxidation process 4. the protective effect of PU depends on the moment of its administration, the best timé being rrioment zero of feperfusipn 5 Its efifect 15 nbt dependent on dose in the range of treatment used | en_US |
dc.format.mimetype | application/pdf | es_ES |
dc.language | cat-en-es | es_ES |
dc.rights | spa | es_ES |
dc.rights | Copyright information available at source archive | es_ES |
dc.subject | none | es_ES |
dc.title | Efecto del tratamiento con pentoxifilina en la isquemia-reperfusión intestinal. | es_ES |
dc.type | doctoral thesis | es_ES |