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Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione

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Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione

dc.contributor.author	Zaragozá Colom, Rosa
dc.contributor.author	García de Mier, Concepción
dc.contributor.author	Rus, Ariana Diana
dc.contributor.author	Pallardó, Federico V.
dc.contributor.author	Barber Sanchis, Teresa
dc.contributor.author	Torres Asensi, Luis
dc.contributor.author	Miralles Fernández, Vicente
dc.contributor.author	Viña Ribes, Juan
dc.date.accessioned	2010-08-10T10:52:36Z
dc.date.available	2010-08-10T10:52:36Z
dc.date.issued	2003
dc.identifier.citation	Zaragozá, R.; García, C.; Rus, A.D.; Pallardó, F.V.; Barber, T.; Torres, L.; Miralles, J.V.; Viña J.R. (2003). Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione. En Biochemical Journal, 373, 825–834	en
dc.identifier.uri	http://hdl.handle.net/10550/16807
dc.description.abstract	In the lactatingmammary gland, weaning produces mitochondrial cytochrome c release and nuclear DNA fragmentation, as determined by gel electrophoresis. This is followed by a significant decrease in lactation. Weaning for 2 h produces an early induction of the tumour suppressor/transcription factor p53, whereas the oncoprotein c-Jun and c-Jun N-terminal kinase are elevated after 24 h of weaning when compared with controls. The expression of p21cip1 and p27kip1, cyclin-dependent kinase inhibitors, was significantly higher in weaned rats when compared with control lactating rats. All the changes mentioned above also happen in the lactatingmammary gland when propargylglycine, an inhibitor of the liver trans-sulphuration pathway, is administered. This effect is partially reversed by N-acetylcysteine administration. The administration of buthionine sulphoximine, an irreversible inhibitor of γ -glutamylcysteine synthetase, to lactating rats produces a decrease in GSH levels and changes in protein concentrations and gene transcripts similar to those in rats with impaired trans-sulphuration pathway. These data suggest that the inter-tissue flux of GSH is an important mechanism of Lcysteine delivery to the lactating mammary gland and emphasize the importance of this physiological event inmaintaining the gene expression required to sustain lactation.	en
dc.language.iso	en	en
dc.subject	apoptosis	en
dc.subject	γ-cystathionase	en
dc.subject	l-cysteine	en
dc.subject	glutathione (GSH)	en
dc.subject	lactation	en
dc.title	Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione	en
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS DE LA VIDA::Bioquímica	en
dc.identifier.doi	10.1042/BJ20030387
dc.identifier.idgrec	000650	en
dc.type.hasVersion	VoR	es_ES
dc.identifier.url	http://www.biochemj.org/bj/373/0825/3730825.pdf	en