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Functional and conformational properties of the exclusive C-domain from the Arabidopsis copper chaperone (CCH)

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Functional and conformational properties of the exclusive C-domain from the Arabidopsis copper chaperone (CCH)

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dc.contributor.author Mira Aparicio, Helena
dc.contributor.author Vilar, Marçal
dc.contributor.author Pérez Payá, Enrique
dc.contributor.author Peñarrubia Blasco, Lola
dc.date.accessioned 2011-06-02T09:26:42Z
dc.date.available 2011-06-02T09:26:42Z
dc.date.issued 2001
dc.identifier.citation Mira Aparicio, Helena; Vilar, Marçal; Perez Paya, Enrique; Peñarrubia Blasco, Dolores. Functional and conformational properties of the exclusive C-domain from the Arabidopsis copper chaperone (CCH. En Biochemical Journal (2001) 357, 545–549 en
dc.identifier.uri http://hdl.handle.net/10550/18828
dc.description.abstract The Arabidopsis thaliana copper chaperone (CCH) is a small copper binding protein involved in copper trafficking. When compared to homologues from other eukaryotes, CCH has two different domains; the conserved N-domain and the plant-exclusive C-domain, a C-terminal extension with an unusual amino-acid composition. In order to characterize this extra C-domain, the CCH protein, the N-domain and the C-domain were all expressed separately in heterologous systems. While the N-domain retained the copper chaperone and antioxidant properties described for the yeast Atx1 and human HAH1 counterparts, the C-domain displayed particular structural properties that would be necessary to optimize copper homoeostasis in plant cells where it could be responsible for the metallochaperone plant-exclusive intercellular transport. The whole CCH protein and the C-domain, but not the N-domain, displayed altered SDS/PAGE mobilities. CD spectroscopy showed that the N-domain fold is representative of an a/b protein, while the C-domain adopts an extended conformation. en
dc.language.iso en en
dc.subject Metallochaperone; Extended structure; Protein domain en
dc.title Functional and conformational properties of the exclusive C-domain from the Arabidopsis copper chaperone (CCH) en
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Bioquímica en
dc.type.hasVersion VoR es_ES
dc.identifier.url http://www.biochemj.org/bj/357/0545/3570545.pdf en

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