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Spontaneous Cardiomyocyte Differentiation From Adipose Tissue Stroma Cells

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Spontaneous Cardiomyocyte Differentiation From Adipose Tissue Stroma Cells

dc.contributor.author	Planat-Bénard, V.
dc.contributor.author	Menard, C.
dc.contributor.author	André, M.
dc.contributor.author	Puceat, M.
dc.contributor.author	Perez, A.
dc.contributor.author	García Verdugo, José Manuel
dc.contributor.author	Pénicaud, L.
dc.contributor.author	Casteilla, L.
dc.date.accessioned	2011-08-19T09:14:19Z
dc.date.available	2011-08-19T09:14:19Z
dc.date.issued	2004
dc.identifier.citation	PLANAT-BÉNARD, V. ; Menard, C. ; André, M. ; Puceat, M. ; Perez, A. ; Garcia Verdugo, Jose Manuel ; Pénicaud, L. ; Casteilla, L., 2004, Spontaneous Cardiomyocyte Differentiation From Adipose Tissue Stroma Cells, Circulation Research, vol. 94, no. 2, p. 223-229	en
dc.identifier.uri	http://hdl.handle.net/10550/20053
dc.description.abstract	Cardiomyocyte regeneration is limited in adult life. Thus, the identification of a putative source of cardiomyocyte progenitors is of great interest to provide a usable model in vitro and new perspective in regenerative therapy. As adipose tissues were recently demonstrated to contain pluripotent stem cells, the emergence of cardiomyocyte phenotype from adipose-derived cells was investigated. We demonstrated that rare beating cells with cardiomyocyte features could be identified after culture of adipose stroma cells without addition of 5-azacytidine. The cardiomyocyte phenotype was first identified by morphological observation, confirmed with expression of specific cardiac markers, immunocytochemistry staining, and ultrastructural analysis, revealing the presence of ventricle- and atrial-like cells. Electrophysiological studies performed on early culture revealed a pacemaker activity of the cells. Finally, functional studies showed that adrenergic agonist stimulated the beating rate whereas cholinergic agonist decreased it. Taken together, this study demonstrated that functional cardiomyocyte- like cells could be directly obtained from adipose tissue. According to the large amount of this tissue in adult mammal, it could represent a useful source of cardiomyocyte progenitors.	en
dc.language.iso	en	en
dc.subject	Cardiomyocytes ; Adipose tissue ; Differentiation ; Stem cells ; Cell therapy	en
dc.title	Spontaneous Cardiomyocyte Differentiation From Adipose Tissue Stroma Cells	en
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	en
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS ::Medicina interna	en
dc.identifier.doi	10.1161/01.RES.0000109792.43271.47	en
dc.identifier.idgrec	010606	en
dc.type.hasVersion	VoR	es_ES
dc.identifier.url	http://circres.ahajournals.org/cgi/reprint/94/2/223	en