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Angiotensin II Induces Leukocyte–Endothelial Cell Interactions In Vivo Via AT1 and AT2 Receptor–Mediated P-Selectin Upregulation

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Angiotensin II Induces Leukocyte–Endothelial Cell Interactions In Vivo Via AT1 and AT2 Receptor–Mediated P-Selectin Upregulation

dc.contributor.author	Piqueras Ruiz, Laura
dc.contributor.author	Kubes, Paul
dc.contributor.author	Álvarez Ribelles, Ángeles
dc.contributor.author	O'Connor Blasco, José Enrique
dc.contributor.author	Issekutz, Andrew C.
dc.contributor.author	Esplugues Mota, Juan Vicente
dc.contributor.author	Sanz Ferrando, María Jesús
dc.date.accessioned	2011-08-19T09:17:10Z
dc.date.available	2011-08-19T09:17:10Z
dc.date.issued	2000
dc.identifier.citation	PIQUERAS RUIZ, Laura ; Kubes, Paul ; Alvarez Ribelles, Angeles ; O'Connor, Enrique ; Issekutz, Andrew C. ; Esplugues Mota, Juan Vicente ; Sanz Ferrando, Maria Jesus, 2000, Angiotensin II Induces Leukocyte–Endothelial Cell Interactions In Vivo Via AT1 and AT2 Receptor–Mediated P-Selectin Upregulation, Circulation, vol. 102, no. 17, p. 2118-2123	en
dc.identifier.uri	http://hdl.handle.net/10550/20058
dc.description.abstract	Background—Angiotensin II (Ang II) plays a critical role in the development of vascular lesions in hypertension, atherosclerosis, and several renal diseases. Because Ang II may contribute to the leukocyte recruitment associated with these pathological states, the aim of the present study was to assess the role of Ang II in leukocyte–endothelial cell interactions in vivo. Methods and Results—Intravital microscopy of the rat mesenteric postcapillary venules was used. Sixty minutes of superfusion with 1 nmol/L Ang II induced a significant increase in leukocyte rolling flux (83.8±20.7 versus 16.4±3.1 cells/min), adhesion (11.4±1.0 versus 0.8±0.5 cells/100 µm), and emigration (4.0±0.7 versus 0.2±0.2 cells/field) without any vasoconstrictor activity. These effects were not mediated by mast cell activation. Intravenous pretreatment with AT1 (losartan) or AT2 (PD123,319) receptor antagonists significantly reduced Ang II–induced responses. A combination of both receptor antagonists inhibited the leukocyte rolling flux, adhesion, and extravasation elicited by Ang II at 60 minutes. Pretreatment of animals with fucoidin or an adhesion-blocking anti–rat P-selectin monoclonal antibody abolished Ang II–induced leukocyte responses. Furthermore, rat platelet P-selectin expression was not affected by Ang II stimulation. Conclusions—Ang II induces significant leukocyte rolling, adhesion, and emigration, which may contribute not only to hypertension but also to the onset and progression of the vascular damage associated with disease states in which plasma levels of this peptide are elevated.	en
dc.language.iso	en	en
dc.subject	Angiotensin ; Endothelium ; Leukocytes ; Cell adhesion molecules ; Glycoproteins	en
dc.title	Angiotensin II Induces Leukocyte–Endothelial Cell Interactions In Vivo Via AT1 and AT2 Receptor–Mediated P-Selectin Upregulation	en
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	en
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS ::Medicina interna	en
dc.identifier.idgrec	003711	en
dc.type.hasVersion	VoR	es_ES
dc.identifier.url	http://circ.ahajournals.org/cgi/reprint/102/17/2118	en