NAGIOS: RODERIC FUNCIONANDO

Topoisomerase II regulates yeast genes with singular chromatin architectures

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Topoisomerase II regulates yeast genes with singular chromatin architectures

dc.contributor.author	Nikolaou, C.
dc.contributor.author	Bermúdez, Ignacio
dc.contributor.author	Manichanh, C.
dc.contributor.author	García Martínez, José
dc.contributor.author	Guigó, R.
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Roca, Joaquim
dc.date.accessioned	2013-11-11T09:18:02Z
dc.date.available	2013-11-11T09:18:02Z
dc.date.issued	2013
dc.identifier.citation	Nikolaou, C.; Bermúdez, Ignacio; Manichanh, C.; García-Martínez, José; Guigó, R.; Pérez-Ortín, José E.; Roca, J.(2013) Topoisomerase II regulates yeast genes with singular chromatin architectures Nucleic Acids Research 41 20 9243 9256
dc.identifier.uri	http://hdl.handle.net/10550/30739
dc.description.abstract	Eukaryotic topoisomerase II (topo II) is the essential decatenase of newly replicated chromosomes and the main relaxase of nucleosomal DNA. Apart from these general tasks, topo II participates in more specialized functions. In mammals, topo IIa interacts with specific RNA polymerases and chromatin-remodeling complexes, whereas topo IIb regulates developmental genes in conjunction with chromatin remodeling and heterochromatin transitions. Here we show that in budding yeast, topo II regulates the expression of specific gene subsets. To uncover this, we carried out a genomic transcription run-on shortly after the thermal inactivation of topo II. We identified a modest number of genes not involved in the general stress response but strictly dependent on topo II. These genes present distinctive functional and structural traits in comparison with the genome average. Yeast topo II is a positive regulator of genes with well-defined promoter architecture that associates to chromatin remodeling complexes; it is a negative regulator of genes extremely hypo-acetylated with complex promoters and undefined nucleosome positioning, many of which are involved in polyamine transport. These findings indicate that yeast topo II operates on singular chromatin architectures to activate or repress DNA transcription and that this activity produces functional responses to ensure chromatin stability.
dc.relation.ispartof	Nucleic Acids Research, 2013, vol. 41, num. 20, p. 9243-9256
dc.subject	Genètica
dc.subject	Genètica molecular
dc.subject	Bioquímica
dc.title	Topoisomerase II regulates yeast genes with singular chromatin architectures
dc.type	journal article	es_ES
dc.date.updated	2013-11-11T09:18:02Z
dc.identifier.doi	10.1093/nar/gkt707
dc.identifier.idgrec	091336
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1093/nar/gkt707