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dc.contributor.author | Novella del Campo, Susana | |
dc.contributor.author | Dantas, A. P. | |
dc.contributor.author | Segarra Irles, Gloria Vicenta | |
dc.contributor.author | Novensá, L. | |
dc.contributor.author | Bueno, C. | |
dc.contributor.author | Heras, Magda | |
dc.contributor.author | Hermenegildo, Carlos | |
dc.contributor.author | Medina Bessó, Pascual | |
dc.date.accessioned | 2013-11-18T11:42:52Z | |
dc.date.available | 2013-11-18T11:42:52Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Novella del Campo, Susana; Dantas, A. P.; Segarra Irles, Gloria; Novensá, L.; Bueno, C.; Heras, M.; Hermenegildo Caudevilla, Carlos; Medina Bessó, Pascual (2013) Aging enhances contraction to thromboxane A2 in aorta from female senescence mice. Age 35 117 128 | |
dc.identifier.uri | http://hdl.handle.net/10550/31106 | |
dc.description.abstract | The time-course for aging-associated effects on vascular reactivity to U46619, a stable analogue of thromboxane A2 (TXA2), was studied in aorta from female senescence-accelerated mice-prone (SAMP8), a murine model of accelerated senescence. SAMP8 and senescence-accelerated mice-resistant (SAMR1) were divided into three groups: 3-, 6- and 10-month-old. Contractile curves to U46619 (10−9 to 10−6 M) were performed in aortic rings in the absence or in the presence of nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME; 10−4 M) and/or cyclooxygenase (COX) inhibitor indomethacin (10−5 M). Protein and gene expression for COX-1 and COX-2 were determined by immunofluorescence and real-time PCR, respectively. Maximal contraction to U46619 was markedly higher in SAMP8 at all ages. In SAMR1, increases were seen at 10 months, while SAMP8 displays augmented contraction at 6 months, which was further increased at 10 months. L-NAME enhanced U46619 contractions in both 6-month-old groups, although the increase was higher on vessels from SAMR1 at this age. Indomethacin equally increased U46619 contractions in both 3-month-old groups, suggesting the production of vasodilator prostaglandin in young animals. In contrast, at 6 and 10 months indomethacin decreased U46619 contractions in both groups, indicating an aging-associated swap to a release of contractile prostanoids in aorta. In conclusion, aging enhances contractile responses to TXA2 in aorta from female mice by a mechanism involving a decrease of NO production and increased action of contractile prostanoids. This process occurs earlier in SAMP8 mice, establishing these mice as good model to study cardiovascular aging in a convenient and standard time-course. | |
dc.relation.ispartof | Age, 2013, vol. 35, p. 117-128 | |
dc.subject | Farmacologia | |
dc.subject | Medicina | |
dc.title | Aging enhances contraction to thromboxane A2 in aorta from female senescence mice | |
dc.type | journal article | es_ES |
dc.date.updated | 2013-11-18T11:42:52Z | |
dc.identifier.doi | 10.1007/s11357-011-9337-y | |
dc.identifier.idgrec | 084861 | |
dc.rights.accessRights | open access | es_ES |
dc.identifier.url | 10.1007/s11357-011-9337-y |