NAGIOS: RODERIC FUNCIONANDO

Study of Spanish Grape Mycobiota and Ochratoxin A Production by the Isolates of Aspergillus tubingensis and other Aspergillus section Nigri

Repositori DSpace/Manakin

Valencià Castellano

Inici
Investigació
e-prints
23 - Química
Visualitza element

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Study of Spanish Grape Mycobiota and Ochratoxin A Production by the Isolates of Aspergillus tubingensis and other Aspergillus section Nigri

Mostra el registre parcial de l'element

dc.contributor.author	Medina Vaya, Angel
dc.contributor.author	Mateo Castro, Rufino
dc.contributor.author	López-Ocaña, Laura
dc.contributor.author	Valle Algarra, Francisco Manuel
dc.contributor.author	Jiménez Escamilla, Misericordia
dc.date.accessioned	2013-11-26T11:55:01Z
dc.date.available	2013-11-26T11:55:01Z
dc.date.issued	2005
dc.identifier.citation	Medina, Angel Mateo Castro, Rufino López-Ocaña, Laura Valle-Algarra, Francisco Manuel Jiménez Escamilla, Misericordia 2005 Study of Spanish Grape Mycobiota and Ochratoxin A Production by the Isolates of Aspergillus tubingensis and other Aspergillus section Nigri Applied and Environmental Microbiology 71 8 4696 4702
dc.identifier.uri	http://hdl.handle.net/10550/31430
dc.description.abstract	The native mycobiota of five grape varieties grown in Spain has been studied. Four (Bobal, Tempranillo, Garnacha, and Monastrell) were red varieties and one (Moscatel) was white. The main fungal genera isolated were Alternaria, Cladosporium, and Aspergillus. The isolation frequency of Aspergillus spp. section Nigri in contaminated samples was 82%. Ochratoxin A (OTA) production was assessed using yeast extract-sucrose broth supplemented with 5% bee pollen. Cultures of 205 isolates from this section showed that 74.2% of Aspergillus carbonarius and 14.3% of Aspergillus tubingensis isolates produced OTA at levels ranging from 1.2 to 3,530 ng/ml and from 46.4 to 111.5 ng/ml, respectively. No Aspergillus niger isolate had the ability to produce this toxin under the conditions assayed. Identification of the A. niger aggregate isolates was based on PCR amplification of 5.8S rRNA genes and its two intergenic spacers, internal transcribed spacer 1 (ITS1) and ITS2, followed by digestion with restriction endonuclease RsaI of the PCR products. The restriction patterns were compared with those from strains of A. niger CECT 2807 and A. tubingensis CECT 20393, held at the Spanish Collection of Type Cultures. DNA sequencing of the ITS1-5.8S rRNA gene-ITS2 region of the OTA-producing isolates of A. tubingensis matched 99 to 100% with the nucleotide sequence of strain A. tubingensis CBS 643.92. OTA determination was accomplished by liquid chromatography with fluorescence detection. OTA confirmation was carried out by liquid chromatography coupled to ion trap mass spectrometry. The results showed that there are significant differences with regard to the isolation frequency of ochratoxinogenic fungi in the different grape varieties. These differences were uncorrelated to berry color. The ability of A. tubingensis to produce OTA and the influence of grape variety on the occurrence of OTA-producing fungi in grapes are described in this report for the first time.
dc.relation.ispartof	Applied and Environmental Microbiology, 2005, vol. 71, num. 8, p. 4696-4702
dc.subject	Microbiologia
dc.subject	Fongs
dc.title	Study of Spanish Grape Mycobiota and Ochratoxin A Production by the Isolates of Aspergillus tubingensis and other Aspergillus section Nigri
dc.type	journal article	es_ES
dc.date.updated	2013-11-26T11:55:01Z
dc.identifier.doi	10.1128/AEM.71.8.4696-4702.2005
dc.identifier.idgrec	014747
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1128/AEM.71.8.4696-4702.2005