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Heat shock response in yeast involver changes in both transcription rates and mRNA stabilities

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Heat shock response in yeast involver changes in both transcription rates and mRNA stabilities

dc.contributor.author	Castells-Roca, L.
dc.contributor.author	García Martínez, José
dc.contributor.author	Moreno Mariño, Joaquín
dc.contributor.author	Herrero, Enrique
dc.contributor.author	Bellí, G.
dc.contributor.author	Pérez Ortín, José Enrique
dc.date.accessioned	2013-11-30T11:45:37Z
dc.date.available	2013-11-30T11:45:37Z
dc.date.issued	2011
dc.identifier.citation	Castells-Roca, L. García-Martínez, J. Moreno Mariño, Joaquín Herrero, E. Bellí, G. Pérez Ortín, José Enrique 2011 Heat shock response in yeast involver changes in both transcription rates and mRNA stabilities Plos One 6 2 e17272
dc.identifier.uri	http://hdl.handle.net/10550/31672
dc.description.abstract	We have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25ºC to 37ºC. Using an established mathematical algorithm, theoretical mRNA decay rates have also been calculated from the experimental data. We have verified the mathematical predictions for selected genes by determining their mRNA decay rates at different times during heat stress response using the regulatable tetO promoter. This study indicates that the yeast response to heat shock is not only due to changes in transcription rates, but also to changes in the mRNA stabilities. mRNA stability is affected in 62% of the yeast genes and it is particularly important in shaping the mRNA profile of the genes belonging to the environmental stress response. In most cases, changes in transcription rates and mRNA stabilities are homodirectional for both parameters, although some interesting cases of antagonist behavior are found. The statistical analysis of gene targets and sequence motifs within the clusters of genes with similar behaviors shows that both transcriptional and post-transcriptional regulons apparently contribute to the general heat stress response by means of transcriptional factors and RNA binding proteins.
dc.relation.ispartof	Plos One, 2011, vol. 6, num. 2, p. e17272
dc.subject	Genètica molecular
dc.subject	Saccharomyces cerevisiae
dc.title	Heat shock response in yeast involver changes in both transcription rates and mRNA stabilities
dc.type	journal article	es_ES
dc.date.updated	2013-11-30T11:45:37Z
dc.identifier.doi	10.1371/journal.pone.0017272
dc.identifier.idgrec	063117
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1371/journal.pone.0017272