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Nonsense-mediated mRNA decay controls the changes in yeast ribosomal protein pre-mRNAs levels upon osmotic stress

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Nonsense-mediated mRNA decay controls the changes in yeast ribosomal protein pre-mRNAs levels upon osmotic stress

dc.contributor.author	Garré García, Elena
dc.contributor.author	Romero Santacreu, Lorena
dc.contributor.author	Barneo Muñoz, Manuela
dc.contributor.author	Miguel, Ana
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Alepuz Martínez, Paula
dc.date.accessioned	2013-12-03T09:05:16Z
dc.date.available	2013-12-03T09:05:16Z
dc.date.issued	2013
dc.identifier.citation	Garré García, Elena; Romero Santacreu, Lorena; Barneo Muñoz, Manuela; Miguel, Ana; Pérez Ortín, José Enrique; Alepuz Martínez, Paula (2013) Nonsense-mediated mRNA decay controls the changes in yeast ribosomal protein pre-mRNAs levels upon osmotic stress Plos One 8 4 e61240
dc.identifier.uri	http://hdl.handle.net/10550/31694
dc.description.abstract	The expression of ribosomal protein (RP) genes requires a substantial part of cellular transcription, processing and translation resources. Thus, the RP expression must be tightly regulated in response to conditions that compromise cell survival. In Saccharomyces cerevisiae cells, regulation of the RP gene expression at the transcriptional, mature mRNA stability and translational levels during the response to osmotic stress has been reported. Reprogramming global protein synthesis upon osmotic shock includes the movement of ribosomes from RP transcripts to stress-induced mRNAs. Using tiling arrays, we show that osmotic stress yields a drop in the levels of RP pre-mRNAs in S. cerevisiae cells. An analysis of the tiling array data, together with transcription rates data, shows a poor correlation, indicating that the drop in the RP pre-mRNA levels is not merely a result of the lowered RP transcription rates. A kinetic study using quantitative RT-PCR confirmed the decrease in the levels of several RP-unspliced transcripts during the first 15 minutes of osmotic stress, which seems independent of MAP kinase Hog1. Moreover, we found that the mutations in the components of the nonsense-mediated mRNA decay (NMD), Upf1, Upf2, Upf3 or in exonuclease Xrn1, eliminate the osmotic stress-induced drop in RP pre-mRNAs. Altogether, our results indicate that the degradation of yeast RP unspliced transcripts by NMD increases during osmotic stress, and suggest that this might be another mechanism to control RP synthesis during the stress response.
dc.relation.ispartof	Plos One, 2013, vol. 8, num. 4, p. e61240
dc.subject	Genètica molecular
dc.subject	Saccharomyces cerevisiae
dc.subject	Saccharomyces
dc.subject	Proteïnes
dc.subject	Estrès oxidatiu
dc.title	Nonsense-mediated mRNA decay controls the changes in yeast ribosomal protein pre-mRNAs levels upon osmotic stress
dc.type	journal article	es_ES
dc.date.updated	2013-12-03T09:05:16Z
dc.identifier.doi	10.1371/journal.pone.0061240
dc.identifier.idgrec	084575
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1371/journal.pone.0061240