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A complete set of nascent transcription rates for yeast genes

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A complete set of nascent transcription rates for yeast genes

dc.contributor.author	Pelechano García, Vicente José
dc.contributor.author	Chávez, Sebastián
dc.contributor.author	Pérez Ortín, José Enrique
dc.date.accessioned	2013-12-04T11:52:17Z
dc.date.available	2013-12-04T11:52:17Z
dc.date.issued	2010
dc.identifier.citation	Pelechano García, Vicente José Chávez, Sebastián Pérez Ortín, José Enrique 2010 A complete set of nascent transcription rates for yeast genes Plos One 5 11 e15442
dc.identifier.uri	http://hdl.handle.net/10550/31732
dc.description.abstract	The amount of mRNA in a cell is the result of two opposite reactions: transcription and mRNA degradation. These reactions are governed by kinetics laws, and the most regulated step for many genes is the transcription rate. The transcription rate, which is assumed to be exercised mainly at the RNA polymerase recruitment level, can be calculated using the RNA polymerase densities determined either by run-on or immunoprecipitation using specific antibodies. The yeast Saccharomyces cerevisiae is the ideal model organism to generate a complete set of nascent transcription rates that will prove useful for many gene regulation studies. By combining genomic data from both the GRO (Genomic Run-on) and the RNA pol ChIP-on-chip methods we generated a new, more accurate nascent transcription rate dataset. By comparing this dataset with the indirect ones obtained from the mRNA stabilities and mRNA amount datasets, we are able to obtain biological information about posttranscriptional regulation processes and a genomic snapshot of the location of the active transcriptional machinery. We have obtained nascent transcription rates for 4,670 yeast genes. The median RNA polymerase II density in the genes is 0.078 molecules/kb, which corresponds to an average of 0.096 molecules/gene. Most genes have transcription rates of between 2 and 30 mRNAs/hour and less than 1% of yeast genes have >1 RNA polymerase molecule/gene. Histone and ribosomal protein genes are the highest transcribed groups of genes and other than these exceptions the transcription of genes is an infrequent phenomenon in a yeast cell.
dc.relation.ispartof	Plos One, 2010, vol. 5, num. 11, p. e15442
dc.subject	Genòmica
dc.subject	Genètica molecular
dc.subject	RNA
dc.subject	Saccharomyces
dc.subject	Transcripció genètica
dc.title	A complete set of nascent transcription rates for yeast genes
dc.type	journal article	es_ES
dc.date.updated	2013-12-04T11:52:17Z
dc.identifier.doi	10.1371/journal.pone.0015442
dc.identifier.idgrec	060545
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1371/journal.pone.0015442