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A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding

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A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding

dc.contributor.author	Bermúdez, Ignacio
dc.contributor.author	García Martínez, José
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Roca, Joaquim
dc.date.accessioned	2013-12-04T12:31:27Z
dc.date.available	2013-12-04T12:31:27Z
dc.date.issued	2010
dc.identifier.citation	Bermúdez, Ignacio García Martínez, José Pérez Ortín, José Enrique Roca, Joaquim 2010 A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding Nucleic Acids Research 38 19 e182
dc.identifier.uri	http://hdl.handle.net/10550/32047
dc.description.abstract	The helical tension of chromosomal DNA is one of the epigenetic landmarks most difficult to examine experimentally. The occurrence of DNA crosslinks mediated by psoralen photobinding (PB) stands as the only suitable probe for assessing this problem. PB is affected by chromatin structure when is done to saturation; but it is mainly determined by DNA helical tension when it is done to very low hit conditions. Hence, we developed a method for genomewide analysis of DNA helical tension based on PB. We adjusted in vitro PB conditions that discern DNA helical tension and applied them to Saccharomyces cerevisiae cells. We selected the in vivo cross-linked DNA sequences and identified them on DNA arrays. The entire procedure was robust. Comparison of PB obtained in vivo with that obtained in vitro with naked DNA revealed that numerous chromosomal regions had deviated PB values. Similar analyses in yeast topoisomerase mutants uncovered further PB alterations across specific chromosomal domains. These results suggest that distinct chromosome compartments might confine different levels of DNA helical tension in yeast. Genome-wide analysis of psoralen<br>DNA PB can be, therefore, a useful approach to uncover a trait of the chromosome architecture not amenable to other techniques.
dc.relation.ispartof	Nucleic Acids Research, 2010, vol. 38, num. 19, p. e182
dc.subject	Genòmica
dc.subject	Genètica molecular
dc.subject	ADN
dc.title	A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding
dc.type	journal article	es_ES
dc.date.updated	2013-12-04T12:31:27Z
dc.identifier.doi	10.1093/nar/gkq687
dc.identifier.idgrec	060544
dc.rights.accessRights	open access	es_ES