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The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors.

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The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors.

dc.contributor.author	Rodríguez-Gil, Alfonso
dc.contributor.author	García Martínez, José
dc.contributor.author	Pelechano García, Vicente José
dc.contributor.author	Muñoz-Centeno, María de la Cruz
dc.contributor.author	Geli, Vincent
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Chávez, Sebastián
dc.date.accessioned	2013-12-04T12:56:58Z
dc.date.available	2013-12-04T12:56:58Z
dc.date.issued	2010
dc.identifier.citation	Rodríguez-Gil, Alfonso García Martínez, José Pelechano García, Vicente José Muñoz-Centeno, María de la Cruz Geli, Vincent Pérez Ortín, José Enrique Chávez, Sebastián 2010 The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors. Nucleic Acids Research 38 14 4651 4664
dc.identifier.uri	http://hdl.handle.net/10550/32049
dc.description.abstract	In order to study the intragenic profiles of active transcription, we determined the relative levels of active RNA polymerase II present at the 3′- and 5′-ends of 261 yeast genes by run-on. The results obtained indicate that the 3′/5′ run-on ratio varies among the genes studied by over 12 log2 units. This ratio seems to be an intrinsic characteristic of each transcriptional unit and does not significantly correlate with gene length, G + C content or level of expression. The correlation between the 3′/5′ RNA polymerase II ratios measured by run-on and those obtained by chromatin immunoprecipitation is poor, although the genes encoding ribosomal proteins present exceptionally low ratios in both cases. We detected a subset of elongation-related factors that are important for maintaining the wild-type profiles of active transcription, including DSIF, Mediator, factors related to the methylation of histone H3-lysine 4, the Bur CDK and the RNA polymerase II subunit Rpb9. We conducted a more detailed investigation of the alterations caused by rpb9Δ to find that Rpb9 contributes to the intragenic profiles of active transcription by influencing the probability of arrest of RNA polymerase II.
dc.relation.ispartof	Nucleic Acids Research, 2010, vol. 38, num. 14, p. 4651-4664
dc.subject	RNA
dc.subject	Transcripció genètica
dc.title	The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors.
dc.type	journal article	es_ES
dc.date.updated	2013-12-04T12:56:58Z
dc.identifier.doi	10.1093/nar/gkq215
dc.identifier.idgrec	060543
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1093/nar/gkq215