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There is a steady-state transcriptome in exponentially growing yeast cells

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There is a steady-state transcriptome in exponentially growing yeast cells

dc.contributor.author	Pelechano García, Vicente José
dc.contributor.author	Pérez Ortín, José Enrique
dc.date.accessioned	2013-12-04T13:37:33Z
dc.date.available	2013-12-04T13:37:33Z
dc.date.issued	2010
dc.identifier.citation	Pelechano García, Vicente José. Pérez Ortín, José Enrique 2010 There is a steady-state transcriptome in exponentially growing yeast cells Yeast 27 7 413 422
dc.identifier.uri	http://hdl.handle.net/10550/32050
dc.description.abstract	The growth of yeast cells in batches in glucose-based media is a standard condition in most yeast laboratories. Most gene expression experiments are done by taking this condition as a reference. Presumably, cells are in a stable physiological condition that can be easily reproduced in other laboratories. With this assumption, however, it is necessary to consider that the average amount of the mRNAs per cell for most genes does not change during exponential growth. That is to say, there is a steady-state condition for the transcriptome. However, this has not been rigorously demonstrated to date. In this work we take several cell samples during the exponential phase growth to perform a kinetic study using the genomic run-on (GRO) technique, which allows simultaneous measurement of the amount of mRNA and transcription rate variation at the genomic level. We show here that the steady-state condition is fulﬁlled for almost all the genes during most exponential growth in yeast extract<br>peptone<br>dextrose medium (YPD) and, therefore, that simultaneous measures of the transcription rates and the amounts of mRNA can be used for indirect mRNA stability calculations. With this kinetic approach, we were also able to dermine the relative inﬂuence of the transcription rate and the mRNA stability changes for the mRNA variation for those genes that deviate from the steady state.
dc.relation.ispartof	Yeast, 2010, vol. 27, num. 7, p. 413-422
dc.subject	Genètica molecular
dc.subject	RNA
dc.subject	Transcripció genètica
dc.subject	Saccharomyces
dc.title	There is a steady-state transcriptome in exponentially growing yeast cells
dc.type	journal article	es_ES
dc.date.updated	2013-12-04T13:37:33Z
dc.identifier.doi	10.1002/yea.1768
dc.identifier.idgrec	060542
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1002/yea.1768