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Regulon-specific control of transcription elongation across the yeast genome

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Regulon-specific control of transcription elongation across the yeast genome

dc.contributor.author	Pelechano García, Vicente José
dc.contributor.author	Jimeno-González, S.
dc.contributor.author	Rodríguez-Gil, Alfonso
dc.contributor.author	García Martínez, José
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Chávez, Sebastián
dc.date.accessioned	2013-12-04T13:49:52Z
dc.date.available	2013-12-04T13:49:52Z
dc.date.issued	2009
dc.identifier.citation	Pelechano García, Vicente José Jimeno-González, S. Rodríguez-Gil, Alfonso García Martínez, José Pérez Ortín, José Enrique Chávez, Sebastián 2009 Regulon-specific control of transcription elongation across the yeast genome Plos Genetics 5 8 e1000614
dc.identifier.uri	http://hdl.handle.net/10550/32053
dc.description.abstract	Transcription elongation by RNA polymerase II was often considered an invariant non-regulated process. However, genome-wide studies have shown that transcriptional pausing during elongation is a frequent phenomenon in tightly-regulated metazoan genes. Using a combination of ChIP-on-chip and genomic run-on approaches, we found that the proportion of transcriptionally active RNA polymerase II (active versus total) present throughout the yeast genome is characteristic of some functional gene classes, like those related to ribosomes and mitochondria. This proportion also responds to regulatory stimuli mediated by protein kinase A and, in relation to cytosolic ribosomal-protein genes, it is mediated by the silencing domain of Rap1. We found that this inactive form of RNA polymerase II, which accumulates along the full length of ribosomal protein genes, is phosphorylated in the Ser5 residue of the CTD, but is hypophosphorylated in Ser2. Using the same experimental approach, we show that the in vivo<br>depletion of FACT, a chromatin-related elongation factor, also produces a regulon-specific effect on the expression of the yeast genome. This work demonstrates that the regulation of transcription elongation is a widespread, gene class<br>dependent phenomenon that also affects housekeeping genes.
dc.relation.ispartof	Plos Genetics, 2009, vol. 5, num. 8, p. e1000614
dc.subject	RNA
dc.subject	Transcripció genètica
dc.subject	Genètica molecular
dc.subject	Saccharomyces
dc.title	Regulon-specific control of transcription elongation across the yeast genome
dc.type	journal article	es_ES
dc.date.updated	2013-12-04T13:49:52Z
dc.identifier.doi	10.1371/journal.pgen.1000614
dc.identifier.idgrec	052954
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1371/journal.pgen.1000614