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Genomic run-on evaluates transcription rates for all yeast genes and identifies gene regulatory mechanisms

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Genomic run-on evaluates transcription rates for all yeast genes and identifies gene regulatory mechanisms

dc.contributor.author	García Martínez, José
dc.contributor.author	Aranda Fernández, Agustín
dc.contributor.author	Pérez Ortín, José Enrique
dc.date.accessioned	2013-12-09T08:17:53Z
dc.date.available	2013-12-09T08:17:53Z
dc.date.issued	2004
dc.identifier.citation	Garcia Martinez, José Aranda Fernández, Agustín Pérez Ortín, José Enrique 2004 Genomic run-on evaluates transcription rates for all yeast genes and identifies gene regulatory mechanisms Molecular Cell 15 2 303 313
dc.identifier.uri	http://hdl.handle.net/10550/32058
dc.description.abstract	Most studies of eukaryotic gene regulation have been done looking at mature mRNA levels. Nevertheless, the steady-state mRNA level is the result of two opposing factors: transcription rate (TR) and mRNA degradation. Both can be important points to regulate gene expression. Here we show a new method that combines the use of nylon macroarrays and in vivo radioactive labeling of nascent RNA to quantify TRs, mRNA levels, and mRNA stabilities for all the S. cerevisiae genes. We found that during the shift from glucose to galactose, most genes undergo drastic changes in TR and mRNA stability. However, changes in mRNA levels are less pronounced. Some genes, such as those encoding mitochondrial proteins, are coordinately regulated in mRNA stability behaving as decay regulons. These results indicate that, although TR is the main determinant of mRNA abundance in yeast, modulation of mRNA stability is a key factor for gene regulation.
dc.relation.ispartof	Molecular Cell, 2004, vol. 15, num. 2, p. 303-313
dc.subject	Genòmica
dc.subject	Saccharomyces
dc.subject	Transcripció genètica
dc.title	Genomic run-on evaluates transcription rates for all yeast genes and identifies gene regulatory mechanisms
dc.type	journal article	es_ES
dc.date.updated	2013-12-09T08:17:53Z
dc.identifier.doi	10.1016/j.molcel.2004.06.004
dc.identifier.idgrec	012019
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1016/j.molcel.2004.06.004