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Transcriptional response of Saccharomyces cerevisiae to different nitrogen concentrations during alcoholic fermentation.

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Transcriptional response of Saccharomyces cerevisiae to different nitrogen concentrations during alcoholic fermentation.

dc.contributor.author	Mendes Ferreira, A.
dc.contributor.author	Olmo Muñoz, Marcel·lí del
dc.contributor.author	García Martínez, José
dc.contributor.author	Jiménez Martí, Elena
dc.contributor.author	Mendes Faia, A.
dc.contributor.author	Pérez Ortín, José Enrique
dc.contributor.author	Leão, C.
dc.date.accessioned	2013-12-09T11:44:54Z
dc.date.available	2013-12-09T11:44:54Z
dc.date.issued	2007
dc.identifier.citation	Mendes Ferreira, A.; Olmo Muñoz, Marcel·lí del; García Martínez, José; Jiménez Martí, Elena; Mendes Faia, A.; Pérez Ortín, José; Enrique Leão C. (2007) Transcriptional response of Saccharomyces cerevisiae to different nitrogen concentrations during alcoholic fermentation. Applied and Environmental Microbiology 73 9 3049 3060
dc.identifier.uri	http://hdl.handle.net/10550/32078
dc.description.abstract	Gene expression profiles of a wine strain of Saccharomyces cerevisiae PYCC4072 were monitored during alcoholic fermentations with three different nitrogen supplies: (i) control fermentation (with enough nitrogen to complete sugar fermentation), (ii) nitrogen-limiting fermentation, and (iii) the addition of nitrogen to the nitrogen-limiting fermentation (refed fermentation). Approximately 70% of the yeast transcriptome was altered in at least one of the fermentation stages studied, revealing the continuous adjustment of yeast cells to stressful conditions. Nitrogen concentration had a decisive effect on gene expression during fermentation. The largest changes in transcription profiles were observed when the early time points of the N-limiting and control fermentations were compared. Despite the high levels of glucose present in the media, the early responses of yeast cells to low nitrogen were characterized by the induction of genes involved in oxidative glucose metabolism, including a significant number of mitochondrial associated genes resembling the yeast cell response to glucose starvation. As the N-limiting fermentation progressed, a general downregulation of genes associated with catabolism was observed. Surprisingly, genes encoding ribosomal proteins and involved in ribosome biogenesis showed a slight increase during N starvation; besides, genes that comprise the RiBi regulon behaved distinctively under the different experimental conditions. Here, for the first time, the global response of nitrogen-depleted cells to nitrogen addition under enological conditions is described. An important gene expression reprogramming occurred after nitrogen addition; this reprogramming affected genes involved in glycolysis, thiamine metabolism, and energy pathways, which enabled the yeast strain to overcome the previous nitrogen starvation stress and restart alcoholic fermentation.
dc.relation.ispartof	Applied and Environmental Microbiology, 2007, vol. 73, num. 9, p. 3049-3060
dc.subject	Saccharomyces
dc.subject	Saccharomyces cerevisiae
dc.subject	Transcripció genètica
dc.title	Transcriptional response of Saccharomyces cerevisiae to different nitrogen concentrations during alcoholic fermentation.
dc.type	journal article	es_ES
dc.date.updated	2013-12-09T11:44:54Z
dc.identifier.doi	10.1128/AEM.02754-06
dc.identifier.idgrec	033666
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1128/AEM.02754-06