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A preliminary study in Wistar rats with enniatin : A contaminated feed

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A preliminary study in Wistar rats with enniatin : A contaminated feed

dc.contributor.author	Manyes Font, Lara
dc.contributor.author	Escrivá, Laura
dc.contributor.author	Serrano Serrano, Ana Belén
dc.contributor.author	Rodríguez Carrasco, Yelko
dc.contributor.author	Tolosa, Josefa
dc.contributor.author	Meca, Giuseppe
dc.contributor.author	Font Pérez, Guillermina
dc.date.accessioned	2014-02-11T08:02:09Z
dc.date.available	2014-02-11T08:02:09Z
dc.date.issued	2013
dc.identifier.citation	Manyes, Lara Escrivá, L. Serrano, A.B. Rodríguez-Carrasco, Y. Tolosa, J. Meca, G. Font, G. 2013 A preliminary study in Wistar rats with enniatin A contaminated feed. Toxicology Mechanisms and Methods
dc.identifier.uri	http://hdl.handle.net/10550/32851
dc.description.abstract	A 28-day repeated dose preliminary assay, using enniatin A naturally contaminated feed through microbial fermentation by a Fusarium tricinctum strain, was carried out employing two months-old female Wistar rats as in vivo experimental model. In order to simulate a physiological test of a toxic compound naturally produced by fungi, five treated animals were fed during twenty-eight days with fermented feed. As control group, five rats were fed with standard feed. At the 28th day, blood samples were collected for biochemical analysis and the gastrointestinal tract, liver and kidneys were removed from each rat for enniatin A detection and quantitation. Digesta were collected from stomach, duodenum, jejunum, ileum and colon. Enniatin A present in organs and in biological fluids was analyzed by liquid chromatography-diode array detector (LC-DAD) and confirmed by LC-mass spectrometry linear ion trap (MS-LIT); also several serum biochemical parameters and a histological analysis of the duodenal tract were performed. No adverse effects were found in any treated rat at the enniatin A concentration (20.91 mg/kg bw/day) tested during the 28-day experiment. Enniatin A quantitation in biological fluids ranged from 1.50 to 9.00 mg/kg, whereas in the gastrointestinal organs the enniatin A concentration ranged from 2.50 to 23.00 mg/kg. The high enniatin A concentration found in jejunum liquid and tissue points to them as an absorption area. Finally, two enniatin A degradation products were identified in duodenum, jejunum and colon content, probably produced by gut microflora.
dc.relation.ispartof	Toxicology Mechanisms and Methods, 2013
dc.subject	Models biològics
dc.subject	Cromatografia de líquids
dc.title	A preliminary study in Wistar rats with enniatin : A contaminated feed
dc.type	journal article	es_ES
dc.date.updated	2014-02-11T08:02:09Z
dc.identifier.idgrec	092695
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.3109/15376516.2013.876135