NAGIOS: RODERIC FUNCIONANDO

Host-Nonspecific Iron Acquisition Systems and Virulence in the Zoonotic Serovar of Vibrio vulnificus

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Host-Nonspecific Iron Acquisition Systems and Virulence in the Zoonotic Serovar of Vibrio vulnificus

Mostra el registre parcial de l'element

dc.contributor.author Pajuelo Gámez, David
dc.contributor.author Lee, Chung-Te
dc.contributor.author Roig Molina, Francisco José
dc.contributor.author Lemos Ramos, Manuel Luis
dc.contributor.author Hor, Lien-I.
dc.contributor.author Amaro González, Carmen
dc.date.accessioned 2014-02-26T08:46:42Z
dc.date.available 2014-02-26T08:46:42Z
dc.date.issued 2014
dc.identifier.citation Pajuelo Gámez, David Lee, Chung-Te Roig, Francisco J. Lemos Ramos, Manuel Luis Hor, Lien-I Amaro González, Carmen 2014 Host-Nonspecific Iron Acquisition Systems and Virulence in the Zoonotic Serovar of Vibrio vulnificus Infection and Immunity 82 2 731 744
dc.identifier.uri http://hdl.handle.net/10550/33326
dc.description.abstract The zoonotic serovar of Vibrio vulnificus (known as biotype 2 serovar E) is the etiological agent of human and fish vibriosis. The aim of the present work was to discover the role of the vulnibactin- and hemin-dependent iron acquisition systems in the pathogenicity of this zoonotic serovar under the hypothesis that both are host-nonspecific virulence factors. To this end, we selected three genes for three outer membrane receptors (vuuA, a receptor for ferric vulnibactin, and hupA and hutR, two hemin receptors), obtained single and multiple mutants as well as complemented strains, and tested them in a series of in vitro and in vivo assays, using eels and mice as animal models. The overall results confirm that hupA and vuuA, but not hutR, are host-nonspecific virulence genes and suggest that a third undescribed host-specific plasmid-encoded system could also be used by the zoonotic serovar in fish. hupA and vuuA were expressed in the internal organs of the animals in the first 24 h of infection, suggesting that they may be needed to achieve the population size required to trigger fatal septicemia. vuuA and hupA were sequenced in strains representative of the genetic diversity of this species, and their phylogenies were reconstructed by multilocus sequence analysis of selected housekeeping and virulence genes as a reference. Given the overall results, we suggest that both genes might form part of the core genes essential not only for disease development but also for the survival of this species in its natural reservoir, the aquatic environment.
dc.relation.ispartof Infection and Immunity, 2014, vol. 82, num. 2, p. 731-744
dc.subject Virulència (Microbiologia)
dc.title Host-Nonspecific Iron Acquisition Systems and Virulence in the Zoonotic Serovar of Vibrio vulnificus
dc.type journal article es_ES
dc.date.updated 2014-02-26T08:46:42Z
dc.identifier.idgrec 093676
dc.rights.accessRights open access es_ES

Visualització       (1.534Mb)

Aquest element apareix en la col·lecció o col·leccions següent(s)

Mostra el registre parcial de l'element

Cerca a RODERIC

Cerca avançada

Visualitza

Estadístiques