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Vibrio vulnificus produces quorum sensing signals of the AHL-class

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Vibrio vulnificus produces quorum sensing signals of the AHL-class

dc.contributor.author	Amaro González, Carmen
dc.contributor.author	Roig Molina, Francisco José
dc.contributor.author	Valiente Conejero, Esmeralda
dc.contributor.author	Bruhn, Jesper Bartholin
dc.contributor.author	Gram, Lone
dc.contributor.author	Larsen, Jens Laurits
dc.contributor.author	Nielsen, Kristian Fog
dc.date.accessioned	2014-02-26T09:40:02Z
dc.date.available	2014-02-26T09:40:02Z
dc.date.issued	2009
dc.identifier.citation	Amaro González, Carmen Roig i Molina, Francisco José Valiente Conejero, Esmeralda Bruhn, Jesper Bartholin Gram, Lone Larsen, Jens Laurits Nielsen, Kristian Fog 2009 Vibrio vulnificus produces quorum sensing signals of the AHL-class Fems Microbiology Ecology 69 1 16 26
dc.identifier.uri	http://hdl.handle.net/10550/33332
dc.description.abstract	Vibrio vulnificus is an aquatic pathogenic bacterium that can cause vibriosis in humans and fish. The species is subdivided into three biotypes with the fishvirulent strains belonging to biotype 2. The quorum sensing (QS) phenomenon mediated by furanosyl borate diester or autoinducer 2 (AI-2) has been described in human strains of biotype 1, and here we show that the luxS gene which encodes AI-2 is present in all strains of V. vulnificus regardless of origin, biotype or serovar.In this study, we also demonstrate that V. vulnificus produces QS signals of the acylated homoserine lactone (AHL) class (AI-1). AHLs were detected in strains of biotype 1 and 2 from water, fish and human wound infections but not in strains isolated from human septicaemic cases. The AHL compound was identified as N-butanoyl-homoserine-lactone (C4-HL) by both reporter strains and by HPLChigh-resolution MS. C4-HL was detected when AHL-positive strains were grown in low-nutrient medium [modified sea water yeast extract (MSWYE)] but not in rich media (tryptic soy broth or brain¿heart infusion) and its production was enhanced when blood factors were added to MSWYE. C4-HL was detected in vivo, in eels infected with AHL-positive biotype 2 strains. No known AHL-related gene was detected by PCR or Southern blot suggesting that AHL-related genes in V. vulnificus are different from those found in other Gram-negative bacteria.
dc.relation.ispartof	Fems Microbiology Ecology, 2009, vol. 69, num. 1, p. 16-26
dc.subject	Microbiologia
dc.title	Vibrio vulnificus produces quorum sensing signals of the AHL-class
dc.type	journal article	es_ES
dc.date.updated	2014-02-26T09:40:03Z
dc.identifier.doi	10.1111/j.1574-6941.2009.00691.x
dc.identifier.idgrec	056527
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1111/j.1574-6941.2009.00691.x