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Membrane Integration of Poliovirus 2B Viroporin

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Membrane Integration of Poliovirus 2B Viroporin

dc.contributor.author	Martínez Gil, Luis
dc.contributor.author	Bañó Polo, Manuel
dc.contributor.author	Redondo, Natalia
dc.contributor.author	Sánchez Martínez, Silvia
dc.contributor.author	Nieva, José Luis
dc.contributor.author	Carrasco, Luis
dc.contributor.author	Mingarro Muñoz, Ismael
dc.date.accessioned	2014-03-28T13:42:36Z
dc.date.available	2014-03-28T13:42:36Z
dc.date.issued	2011
dc.identifier.citation	Martínez Gil, Luis Bañó Polo, Manuel Redondo, Natalia Sánchez Martínez, Silvia Nieva, José Luis Carrasco, Luis Mingarro Muñoz, Ismael 2011 Membrane Integration of Poliovirus 2B Viroporin Journal of Virology 85 21 11315 11324
dc.identifier.uri	http://hdl.handle.net/10550/33765
dc.description.abstract	Virus infections can result in a variety of cellular injuries, and these often involve the permeabilization of host membranes by viral proteins of the viroporin family. Prototypical viroporin 2B is responsible for the alterations in host cell membrane permeability that take place in enterovirus-infected cells. 2B protein can be localized at the endoplasmic reticulum (ER) and the Golgi complex, inducing membrane remodeling and the blockade of glycoprotein trafficking. These findings suggest that 2B has the potential to integrate into the ER membrane, but specific information regarding its biogenesis and mechanism of membrane insertion is lacking. Here, we report experimental results of in vitro translation-glycosylation compatible with the translocon-mediated insertion of the 2B product into the ER membrane as a double-spanning integral membrane protein with an N-/C-terminal cytoplasmic orientation. A similar topology was found when 2B was synthesized in cultured cells. In addition, the in vitro translation of several truncated versions of the 2B protein suggests that the two hydrophobic regions cooperate to insert into the ER-derived microsomal membranes.
dc.relation.ispartof	Journal of Virology, 2011, vol. 85, num. 21, p. 11315-11324
dc.subject	Farmacologia
dc.subject	Medicina
dc.title	Membrane Integration of Poliovirus 2B Viroporin
dc.type	journal article	es_ES
dc.date.updated	2014-03-28T13:42:36Z
dc.identifier.doi	10.1128/JVI.05421-11
dc.identifier.idgrec	068268
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1128/JVI.05421-11