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Off-axis Digital Holographic Microscopy: practical design parameters for operating at diffraction limit

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Off-axis Digital Holographic Microscopy: practical design parameters for operating at diffraction limit

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dc.contributor.author	Sánchez Ortiga, Emilio
dc.contributor.author	Doblas Expósito, Ana Isabel
dc.contributor.author	Saavedra Tortosa, Genaro
dc.contributor.author	Martínez Corral, Manuel
dc.contributor.author	García Sucerquia, Jorge Iván
dc.date.accessioned	2014-04-08T10:45:31Z
dc.date.available	2014-04-08T10:45:31Z
dc.date.issued	2014
dc.identifier.citation	Sánchez Ortiga, Emilio Doblas Expósito, Ana Saavedra Tortosa, Genaro Martínez Corral, Manuel Garcia Sucerquia, J.I. 2014 Off-axis Digital Holographic Microscopy: practical design parameters for operating at diffraction limit Applied Optics 53 10 2058 2066
dc.identifier.uri	http://hdl.handle.net/10550/34482
dc.description.abstract	The utilization of microscope objectives (MOs) in digital holographic microscopy (DHM) has associated effects that are not present in conventional optical microscopy. The remaining phase curvature, which can ruin the quantitative phase imaging, is the most evident and analyzed. As phase imaging is considered, this interest has made possible the development of different methods of overcoming its undesired consequences. Additionally to the effects in phase imaging, there exist a set of less obvious conditions that have to be accounted for as MOs are utilized in DHM to achieve diffraction-limit operation. These conditions have to be considered even in the case in which only amplitude or intensity imaging is of interest. In this paper, a thorough analysis of the physical parameters that control the appropriate utilization of MOs in DHM is presented. A regular DHM system is theoretically modeled on the basis of the imaging theory. The Fourier spectrum of the recorded hologram is analyzed to evaluate the performance of the DHM. A set of the criteria that consider the microscope features and the recording parameters to achieve DHM operation at the diffraction limit is derived. Numerical modeling and experimental results are shown to validate our findings.
dc.relation.ispartof	Applied Optics, 2014, vol. 53, num. 10, p. 2058-2066
dc.subject	Òptica
dc.title	Off-axis Digital Holographic Microscopy: practical design parameters for operating at diffraction limit
dc.type	journal article	es_ES
dc.date.updated	2014-04-08T10:45:31Z
dc.identifier.doi	10.1109/JDT.2013.2291110
dc.identifier.idgrec	094500
dc.rights.accessRights	open access	es_ES
dc.identifier.url	10.1109/JDT.2013.2291110