Objectives: An attempt was made to evaluate effect of denture wearing on occurrence of fungal isolates in the oral cavity before and after complete denture insertion. Method: Twenty five completely edentulous patients were selected; swab samples were collected intraorally before fabrication of complete dentures from labial vestibular area and after complete denture fabrication (one and four days after denture insertion). Further these samples were inoculated and incubated. Results: In nineteen patients no isolate of fungus before denture insertion as well as 4 days after denture insertion was found. In two subject results were false positive (contamination from environment), and in four patients there was increase in growth but not much significant increase of growth of fungal isolates was seen (mild growth of fungus only after denture insertion). One of the major finding of this study was overall occurrence of fungal isolates (before and after denture insertion) in the oral cavity were not significant.