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Vibrio vulnificus biotype 2 serovar E gne but not galE is essential for lipopolysaccharide biosynthesis and virulence

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Vibrio vulnificus biotype 2 serovar E gne but not galE is essential for lipopolysaccharide biosynthesis and virulence

dc.contributor.author	Valiente Conejero, Esmeralda
dc.contributor.author	Jiménez, Natalia
dc.contributor.author	Merino, Susana
dc.contributor.author	Tomás, Juan M.
dc.contributor.author	Amaro González, Carmen
dc.date.accessioned	2014-12-29T13:09:59Z
dc.date.available	2014-12-29T13:09:59Z
dc.date.issued	2008
dc.identifier.citation	Valiente Conejero, Esmeralda Jiménez, Natalia Merino, Susana Tomás, Juan M. Amaro González, Carmen 2008 Vibrio vulnificus biotype 2 serovar E gne but not galE is essential for lipopolysaccharide biosynthesis and virulence Infection and Immunity 76 4 1628 1638
dc.identifier.uri	http://hdl.handle.net/10550/41083
dc.description.abstract	Vibrio vulnificus biotype 2 serovar E (VSE) is the aetiological agent of both eel vibriosis epizootics and sporadic cases of human vibriosis. We selected two paralogs for UDP-glucose 4-epimerase, corresponding to annotations VV3026 and VV2639 in the genome of the biotype 1 strain YJ016. We demonstrate that VV3026-like and VV2639-like were functionally homologous to gne and galE, respectively. Both genes were present in all V. vulnificus strains tested regardless of biotype or serovar. Two VSE mutants, 4999-2 (gne-deficient) and 4999-3 (galE-deficient), were obtained by allelic exchange. The 4999-2 mutant showed deep alterations in the outer membrane (OM) architecture concomitantly to a noticeable increase in sensitivity to cationic peptides and serum together with the loss of virulence for eels and mice. In contrast, the mutant 4999-3 did not present alterations in either its OM or in surface hydrophobicity, giving the same results as the wild-type strain in all virulence assays. The change to avirulent phenotype produced by mutation in gne was correlated to the loss of the O-antigen LPS. Complementation of gne-deficient mutant restored the LPS structure together with the virulent phenotype. We also demonstrate that Gne is able to perform UDP-GalNAc 4-epimerase activity, as well as UDP-Gal-4-epimerase (GalE) activity, which explain the results obtained with mutant 4999-3 (galE-deficient).
dc.language.iso	eng
dc.relation.ispartof	Infection and Immunity, 2008, vol. 76, num. 4, p. 1628-1638
dc.subject	Bacteris patògens
dc.title	Vibrio vulnificus biotype 2 serovar E gne but not galE is essential for lipopolysaccharide biosynthesis and virulence
dc.type	journal article	es_ES
dc.date.updated	2014-12-29T13:09:59Z
dc.identifier.doi	10.1128/IAI.01393-07
dc.identifier.idgrec	046473
dc.rights.accessRights	open access	es_ES