Objectives: The objective of this study is to evaluate the efficacy of self and professionally applied desensitizing agents in relieving dentinal hypersensitivity after single direct topical application. Study Design: This was a randomized controlled trial conducted among 57 patients. 8% Arginine paste was self- applied by the subject and Gluma desensitizer was applied by investigator. Numeric rating scale was used to mea - sure hypersensitivity after tactile stimulus, Schiff scale was used for cold and air blast stimuli respectively. Scores were recorded at baseline, immediately, 15 and 30 days after the application. Friedman, Wilcoxon test as post hoc was used to analyze within group differences, between group differences analyzed using Mann Whitney U test ( P <0.05 considered significant). Results: 8% Arginine paste elicited significantly higher reductions in sensitivity ( P <0.05) than that of Gluma group at all follow ups. There was a significant decrease in hypersensitivity for both the groups from baseline till final follow-up ( P <0.05) for all three stimuli. 8% Arginine paste was found to be more effective than Gluma desensitizer in providing immediate relief from dentine hypersensitivity and also sustained the effect significantly for a period of 30 days. Conclusions: Self applied 8% Arginine paste is effective than professionally applied Gluma desensitizer in relie - ving dentinal hypersensitivity immediately and over a period of one month.