Background: A number of techniques are being practiced for the evaluation of VDO, but none of them is scientifi - cally more accurate than other. Each method advocated has its own limitations. Objectives: The purpose of this study was to find correlation between vertical dimension of occlusion (VDO) and interpupillary distance (IPD). Material and Methods: A cross-sectional study was conducted on 400 dentate subjects comprising of 200 males and 200 females. Anthropometric measurement of VDO was recorded clinically using modified digital vernier caliper. Also, a standardized digital photograph of face was generated from the frontal aspect using a digital camera for the measurement of IPD in millimeters. Correlation between VDO and IPD was studied using Spearman's coefficient. For the execution of regression command and preparation of prediction equations to estimate VDO, Statistical Package for Social Sciences (SPSS) Software Version 11.5 was used. Results: VDO and IPD was more in males compared to females. VDO was significantly and positively correlated with IPD only in males whereas females showed a weak correlation. Hence, regression equation was derived only for males. VDO estimation using regression equation for IPD had a standard error of ± 3.94 in males. Conclusions: Since the variations between VDO and IPD are within the range of 2-4 mm, VDO prediction through this method is reliable and reproducible for male patients. Also, the method is simple, economic, and non invasive; hence it could be recommended for everyday practice to determine vertical dimension of occlusion in case of male patients.