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In Candida parapsilosis the ATC1 gene encodes for an acid trehalase involved in trehalose hydrolysis, stress resistance and virulence

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In Candida parapsilosis the ATC1 gene encodes for an acid trehalase involved in trehalose hydrolysis, stress resistance and virulence

dc.contributor.author	Sánchez-Fresneda, R.
dc.contributor.author	Martínez-Esparza, M.
dc.contributor.author	Maicas i Prieto, Sergi
dc.contributor.author	Argüelles, Juan Carlos
dc.contributor.author	Valentín, E.
dc.date.accessioned	2015-02-26T07:47:12Z
dc.date.available	2015-02-26T07:47:12Z
dc.date.issued	2014
dc.identifier.citation	Sánchez-Fresneda, R.; Martínez-Esparza, M.; Maicas i Prieto, Sergi; Argüelles, Juan Carlos; Valentín, E. (2014) In Candida parapsilosis the ATC1 gene encodes for an acid trehalase involved in trehalose hydrolysis, stress resistance and virulence Plos One 9 6 e99113
dc.identifier.uri	http://hdl.handle.net/10550/42307
dc.description.abstract	An ORF named CPAR2-208980 on contig 005809 was identified by screening a Candida parapsilosis genome data base. Its 67% identity with the acid trehalase sequence from C. albicans (ATC1) led us to designate it CpATC1. Homozygous mutants that lack acid trehalase activity were constructed by gene disruption at the two CpATC1 chromosomal alleles. Phenotypic characterization showed that atc1Δ null cells were unable to grow on exogenous trehalose as carbon source, and also displayed higher resistance to environmental challenges, such as saline exposure (1.2 M NaCl), heat shock (42°C) and both mild and severe oxidative stress (5 and 50 mM H2O2). Significant amounts of intracellular trehalose were specifically stored in response to the thermal upshift in both wild type and mutant strains. Analysis of their antioxidant activities revealed that catalase was only triggered in response to heat shock in atc1Δ cells, whereas glutathione reductase was activated upon mild oxidative stress in wild type and reintegrant strains, and in response to the whole set of stress treatments in the homozygous mutant. Furthermore, yeast cells with double CpATC1 deletion were significantly attenuated in non-mammalian infection models, suggesting that CpATC1 is required for the pathobiology of the fungus. Our results demonstrate the involvement of CpAtc1 protein in the physiological hydrolysis of external trehalose in C. parapsilosis, where it also plays a major role in stress resistance and virulence.
dc.language.iso	eng
dc.relation.ispartof	Plos One, 2014, vol. 9, num. 6, p. e99113
dc.subject	Microbiologia
dc.title	In Candida parapsilosis the ATC1 gene encodes for an acid trehalase involved in trehalose hydrolysis, stress resistance and virulence
dc.type	journal article	es_ES
dc.date.updated	2015-02-26T07:47:12Z
dc.identifier.doi	10.1371/journal.pone.0099113
dc.identifier.idgrec	099156
dc.rights.accessRights	open access	es_ES