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Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle

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Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle

dc.contributor.author	Jordán Plá, Antonio
dc.contributor.author	Gupta, I.
dc.contributor.author	De Miguel-Jiménez, Lola
dc.contributor.author	Steinmetz, Lars
dc.contributor.author	Chávez, Sebastián
dc.contributor.author	Pelechano García, Vicente José
dc.contributor.author	Pérez Ortín, José Enrique
dc.date.accessioned	2015-04-27T13:24:32Z
dc.date.available	2015-04-27T13:24:32Z
dc.date.issued	2015
dc.identifier.citation	Jordán Plá, Antonio Gupta, I. Miguel Jiménez, Lola de Steinmetz, L. M. Chávez, Sebastián Pelechano García, Vicente José Pérez Ortín, José Enrique 2015 Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle Nucleic Acids Research 43 2 787 802
dc.identifier.uri	http://hdl.handle.net/10550/43437
dc.description.abstract	The particular behaviour of eukaryotic RNA polymerases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution following a new biotin-based genomic run-on (BioGRO) technique. Compared with conventional profiling with chromatin immunoprecipitation, the analysis of the BioGRO profiles in Saccharomyces cerevisiae shows that RNA polymerase II has unique activity profiles at both gene ends, which are highly dependent on positioned nucleosomes. This is the first demonstration of the in vivo influence of positioned nucleosomes on transcription elongation. The particular features at the 5′ end and around the polyadenylation site indicate that this polymerase undergoes extensive specific-activity regulation in the initial and final transcription elongation phases. The genes encoding for ribosomal proteins show distinctive features at both ends. BioGRO also provides the first nascentome analysis for RNA polymerase III, which indicates that transcription of tRNA genes is poorly regulated at the individual copy level. The present study provides a novel perspective of the transcription cycle that incorporates inactivation/reactivation as an important aspect of RNA polymerase dynamics.
dc.relation.ispartof	Nucleic Acids Research, 2015, vol. 43, num. 2, p. 787-802
dc.subject	RNA
dc.subject	Transcripció genètica
dc.subject	Saccharomyces cerevisiae
dc.title	Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle
dc.type	journal article	es_ES
dc.date.updated	2015-04-27T13:24:33Z
dc.identifier.doi	10.1093/nar/gku1349
dc.identifier.idgrec	101170
dc.rights.accessRights	open access	es_ES