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In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin

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In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin

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dc.contributor.author Solá Ruiz, María Fernanda es
dc.contributor.author Pérez Martínez, Carolina es
dc.contributor.author Martín de Llano, José Javier es
dc.contributor.author Carda Batalla, María del Carmen es
dc.contributor.author Labaig Rueda, Carlos es
dc.date.accessioned 2015-05-08T10:40:52Z
dc.date.available 2015-05-08T10:40:52Z
dc.date.issued 2015 es
dc.identifier.citation Solá Ruiz, María Fernanda ; Pérez Martínez, Carolina ; Martín del Llano, José Javier ; Carda Batalla, María del Carmen ; Labaig Rueda, Carlos. In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin. En: Medicina oral, patología oral y cirugía bucal. Ed inglesa, 2015, Vol. 20, No. 1: 2- es
dc.identifier.uri http://hdl.handle.net/10550/43615
dc.description.abstract Objectives: To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application. Study Design: Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: Group I: discs treated with dual acid etching; Group II dual acid etching and blasting with calcium phosphate particles; Group III (control) machined discs. Surface roughness and topography of the discs were examined with scanning electron microscope (SEM) and confocal laser scanning electron microscope( CLSM). Osteoblast adhesion, proliferation and cell morphology were determined by means of fluorescence microscopy with Image-Pro Plus software and SEM. Results: Group II presented the roughest discs, while the least rough were Group III. Cell adhesion was greatest in Group II. The addition of melatonin improved cell proliferation. Conclusions: 1. Surface treatments (dual acid etching, calcium phosphate impaction) increase surface roughness in comparison with machined titanium. 2. Greater surface roughness tends to favor cell adhesion after 24-hour cell culture. 3. The addition of melatonin tends to favor osteoblast proliferation. en_US
dc.subject Odontología es
dc.subject Ciencias de la salud es
dc.title In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin es
dc.type journal article es_ES
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.type.hasVersion VoR es_ES
dc.identifier.url http://www.medicinaoral.com/pubmed/medoralv20_i1_p2.pdf es

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