NAGIOS: RODERIC FUNCIONANDO

Optimization of buffer solutions to analyze inflammatory cytokines in gingival crevicular fluid by multiplex flow cytometry

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Optimization of buffer solutions to analyze inflammatory cytokines in gingival crevicular fluid by multiplex flow cytometry

dc.contributor.author	Ríos Lugo, María Judith	es
dc.contributor.author	Martín, Conchita	es
dc.contributor.author	Alarcón, Jose Antonio	es
dc.contributor.author	Esquifino Parras, Ana Isabel	es
dc.contributor.author	Barbieri, Germán	es
dc.contributor.author	Solano, Patricia	es
dc.contributor.author	Sanz Alonso, Mariano	es
dc.date.accessioned	2015-05-08T10:40:52Z
dc.date.available	2015-05-08T10:40:52Z
dc.date.issued	2015	es
dc.identifier.citation	Ríos Lugo, María Judith ; Martín, Conchita ; Alarcón, Jose Antonio ; Esquifino Parras, Ana Isabel ; Barbieri, Germán ; Solano, Patricia ; Sanz Alonso, Mariano. Optimization of buffer solutions to analyze inflammatory cytokines in gingival crevicular fluid by multiplex flow cytometry. En: Medicina oral, patología oral y cirugía bucal. Ed inglesa, 2015, Vol. 20, No. 1: 5-	es
dc.identifier.uri	http://hdl.handle.net/10550/43618
dc.description.abstract	Objective: the aim of this study was to test two buffer solutions in order to attain a reliable and reproducible analysis of inflammatory cytokines (IL-1β, IL-6, TNF-α, OPG, OPN and OC), in gingival crevicular fluid (GCF) by flow cytometry. Material and Methods: GCF samples from healthy volunteers were collected with perio-paper strips and diluted either in phosphate buffered saline (PBS) or Tris-HCl buffer, with and without protease inhibitors (PI). Cytokine immunoassays were carried out by flow cytometry (Luminex Xmap 200 ) generating standard curves. Results: standards curves generated with the use of phosphate-buffered saline (PBS) demonstrated best adjustment for cytokines IL-1ß, IL-6 and TNF- α levels, when using Tris-HCl ( p <0.05). Conclusions: The use of PBS buffer with the addition of PI provided reliable measurements of inflammatory biomarkers in GCF samples of healthy volunteers.	en_US
dc.subject	Odontología	es
dc.subject	Ciencias de la salud	es
dc.title	Optimization of buffer solutions to analyze inflammatory cytokines in gingival crevicular fluid by multiplex flow cytometry	es
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	es
dc.type.hasVersion	VoR	es_ES
dc.identifier.url	http://www.medicinaoral.com/pubmed/medoralv20_i1_p5.pdf	es