NAGIOS: RODERIC FUNCIONANDO

Intracellular coexpression of CXC- and CC– chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation

Repositori DSpace/Manakin

IMPORTANT: Aquest repositori està en una versió antiga des del 3/12/2023. La nova instal.lació está en https://roderic.uv.es/

Intracellular coexpression of CXC- and CC– chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation

dc.contributor.author	Pinto, Sandra	es_ES
dc.contributor.author	Martínez-Romero, Alicia	es_ES
dc.contributor.author	O'Connor Blasco, José Enrique	es_ES
dc.contributor.author	Gil-Benso, Rosario	es_ES
dc.contributor.author	San Miguel Díez, Teresa	es_ES
dc.contributor.author	Terrádez Mas, Liria	es_ES
dc.contributor.author	Monteagudo Castro, Carlos	es_ES
dc.contributor.author	Callaghan Pitlik, Robert Carles	es_ES
dc.date.accessioned	2015-06-05T12:40:31Z
dc.date.available	2015-06-05T12:40:31Z
dc.date.issued	2014
dc.identifier.citation	BMC Cancer Vol. 14 pp. 118-118	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44193
dc.description.abstract	BackgroundChemokines have been implicated in tumor progression and metastasis. In melanoma, chemokine receptors have been implicated in organ selective metastasis by regulating processes such as chemoattraction, adhesion and survival.MethodsIn this study we have analyzed, using flow cytometry, the systems formed by the chemokine receptors CXCR3, CXCR4, CXCR7, CCR7 and CCR10 and their ligands in thirteen human melanoma cell lines (five established from primary tumors and eight established from metastasis from different tissues). WM-115 and WM-266.4 melanoma cell lines (obtained from a primary and a metastatic melanoma respectively) were xenografted in nude mice and the tumors and cell lines derived from them were also analyzed.ResultsOur results show that the melanoma cell lines do not express or express in a low degree the chemokine receptors on their cell surface. However, melanoma cell lines show intracellular expression of all the aforementioned receptors and most of their respective ligands. When analyzing the xenografts and the cell lines obtained from them we found variations in the intracellular expression of chemokines and chemokine receptors that differed between the primary and metastatic cell lines. However, as well as in the original cell lines, minute or no expression of the chemokine receptors was observed at the cell surface.ConclusionsCoexpression of chemokine receptors and their ligands was found in human melanoma cell lines. However, this expression is intracellular and receptors are not found at the cell membrane nor chemokines are secreted to the cell medium. The levels of expressed chemokine receptors and their ligands show dynamic variations after xenotransplantation that differ depending on the origin of the cell line (from primary tumor or from metastasis).	es_ES
dc.subject	Melanoma	es_ES
dc.subject	Cell line	es_ES
dc.subject	Chemokine receptor	es_ES
dc.subject	Chemokine	es_ES
dc.subject	Xenotransplantation	es_ES
dc.title	Intracellular coexpression of CXC- and CC– chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation	es_ES
dc.type	journal article	es_ES
dc.identifier.doi	10.1186/1471-2407-14-118	es_ES
dc.identifier.idgrec	095212	es_ES